Effects of strontium on proliferation and differentiation of rat bone marrow mesenchymal stem cells
Effects of strontium on proliferation and differentiation of rat bone marrow mesenchymal stem cells
复制标题
锶对大鼠骨髓间充质干细胞增殖和分化的影响
DOI：
10.1016/j.bbrc.2012.01.088
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发表时间：
2012-02-24
影响因子：
3.1
通讯作者：
Hu, Jing
中科院分区：
文献类型：
--
作者：
Li, Yunfeng;Li, Jihua;Hu, Jing
雷奈酸锶（SrR）是一种有效的抗骨质疏松药物，可增加骨形成，减少骨吸收。然而，关于SR对骨髓间充质干细胞（BMMSCs）向成骨细胞和脂肪细胞分化的影响的报道有限。本研究的目的是评估SrR是否影响BMMSCs向成骨细胞或脂肪细胞分化的能力。通过流式细胞术鉴定大鼠BMMSCs，并将其暴露于成骨或成脂培养基下的SR（0.1和1.0 mM Sr 2+）1周和2周。采用MTT法、碱性磷酸酶（ALP）法、油红0染色法、实时荧光定量RT-PCR法和Western blot法检测BMMSCs的增殖和分化情况。SrR可显著抑制大鼠BMMSCs的增殖，促进其向成骨细胞分化，但降低其向脂肪细胞分化，且呈剂量依赖性。在成骨培养液中，SrR可促进成骨细胞ALP的表达，RT-PCR检测到Cbfa 1/Runx 2、骨涎蛋白和骨钙素的mRNA水平，Western blot检测到Cbfa 1/Runx 2的蛋白水平。在成脂培养液中，SrR通过RT-PCR降低了PPAR γ 2、脂肪细胞脂质结合蛋白2（aP 2/ALBP）和脂蛋白脂酶（LPL）的mRNA水平，通过Western blot分析降低了PPAR γ的蛋白表达。这些结果表明SrR促进BMMSC成骨细胞分化但抑制脂肪细胞分化的作用可能有助于其治疗骨质疏松症的作用。(C)2012 Elsevier Inc. All rights reserved.
Strontium ranelate (SrR) was an effective anti-osteoporotic drug to increase bone formation and decrease bone resorption. However, reports about the effect of SR on osteoblastic and adipocytic differentiation from bone marrow mesenchymal stem cells (BMMSCs) are limited. The purpose of this study is to evaluate whether SrR affects the ability of BMMSCs to differentiate into osteoblasts or adipocytes. Rat BMMSCs were identified by flow cytometry and exposed to SR (0.1 and 1.0 mM Sr2+) under osteogenic or adipogenic medium for 1 and 2 weeks. The proliferation and differentiation of BMMSCs were analyzed by MTT, alkaline phosphatase (ALP), Oil red 0 staining, quantitative real-time RT-PCR and Western blot assays. SrR significantly inhibited the proliferation, increased osteoblastic but decreased adipocytic differentiation of rat BMMSCs dose-dependently. In osteogenic medium, SrR increased the expression of ALP, the mRNA levels of Cbfa1/Runx2, bone sialoprotein, and osteocalcin by RT-PCR, and the protein levels of Cbfa1/Runx2 by Western blot. In adipogenic medium, SrR decreased the mRNA levels of PPAR gamma 2, adipocyte lipid-binding protein 2 (aP2/ALBP), and lipoprotein lipase (LPL) by RT-PCR, and the protein expression of PPAR gamma in Western blot analysis. These results indicated that the effects of SrR to promote osteoblastic but inhibit adipocytic differentiation of BMMSCs might contribute to its effect on osteoporosis treatment. (C) 2012 Elsevier Inc. All rights reserved.