RNA viral community in human feces: prevalence of plant pathogenic viruses.
RNA viral community in human feces: prevalence of plant pathogenic viruses.
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人类粪便中的RNA病毒群落：植物致病病毒的患病率。
DOI：
10.1371/journal.pbio.0040003
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发表时间：
2006-01
期刊：
影响因子：
9.8
通讯作者：
Ruan, Yijun
中科院分区：
文献类型：
--
作者：
Zhang, Tao;Breitbart, Mya;Lee, Wah Heng;Run, Jin-Quan;Wei, Chia Lin;Soh, Shirlena Wee Ling;Hibberd, Martin L;Liu, Edison T;Rohwer, Forest;Ruan, Yijun
众所周知，人类的肠道是包括病毒在内的各种微生物的储藏库。已知许多RNA病毒与胃肠炎有关；然而，健康人中存在的肠道RNA病毒群落尚未被描述。在这里，我们提出了从两个健康人的三个粪便样本中发现的RNA病毒的比较元基因组分析。在本研究中，未培养的病毒通过切向流过滤浓缩，提取病毒RNA并克隆到鸟枪式病毒cDNA文库中进行测序分析。在获得的36,769个病毒序列中，绝大多数与植物致病RNA病毒相似。这项研究中最丰富的粪便病毒是辣椒轻度斑驳病毒(PMMV)，它在高浓度下被发现-每克干重粪便中最多有109个病毒粒子。在采集自两大洲健康人的18份粪便样本中，有12份(66.7%)检出PMMV，表明这种植物病毒在人群中流行。许多以胡椒为基础的食品PMMV检测呈阳性，表明这种病毒的饮食来源。有趣的是，粪便PMMV对寄主植物具有传染性，这表明人类可能是传播某些植物病毒的媒介。对人类肠道中的RNA病毒进行的比较元基因组分析确定，绝大多数是植物病原体。
The human gut is known to be a reservoir of a wide variety of microbes, including viruses. Many RNA viruses are known to be associated with gastroenteritis; however, the enteric RNA viral community present in healthy humans has not been described. Here, we present a comparative metagenomic analysis of the RNA viruses found in three fecal samples from two healthy human individuals. For this study, uncultured viruses were concentrated by tangential flow filtration, and viral RNA was extracted and cloned into shotgun viral cDNA libraries for sequencing analysis. The vast majority of the 36,769 viral sequences obtained were similar to plant pathogenic RNA viruses. The most abundant fecal virus in this study was pepper mild mottle virus (PMMV), which was found in high concentrations—up to 109 virions per gram of dry weight fecal matter. PMMV was also detected in 12 (66.7%) of 18 fecal samples collected from healthy individuals on two continents, indicating that this plant virus is prevalent in the human population. A number of pepper-based foods tested positive for PMMV, suggesting dietary origins for this virus. Intriguingly, the fecal PMMV was infectious to host plants, suggesting that humans might act as a vehicle for the dissemination of certain plant viruses. A comparative metagenomic analysis of RNA viruses in the human gut identifies the vast majority as plant pathogens.