VARIANTS OF HERPES SIMPLEX VIRUS: ISOLATION, CHARACTERIZATION, AND FACTORS INFLUENCING PLAQUE FORMATION
VARIANTS OF HERPES SIMPLEX VIRUS: ISOLATION, CHARACTERIZATION, AND FACTORS INFLUENCING PLAQUE FORMATION
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单纯疱疹病毒变种：分离、表征和影响斑块形成的因素
DOI：
10.1128/jb.86.5.985-991.1963
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发表时间：
1963
影响因子：
3.2
通讯作者：
F. Rapp
中科院分区：
文献类型：
--
作者：
F. Rapp
Rapp，Fred（Baylor University College of Medicine，Houston，Tex.）.单纯疱疹病毒的变异体：分离、表征和影响空斑形成的因素。J. Bacteriol. 86：985-991。1963.-分离出单纯疱疹病毒的变种，它们的噬斑大小和对兔子和小鼠的毒力不同。兔角膜的角膜炎和全身性疾病与大斑块变异有关，而与小斑块变异无关。对于断奶小鼠，大噬斑变异体的神经毒性与亲本病毒株相同，但大多数小噬斑变异体对小鼠的毒性仅为亲本病毒的1%。琼脂可抑制亲本菌株和所有供试变体的噬菌斑形成;噬菌斑发育迟缓，噬菌斑数量比用甲基纤维素替代琼脂的覆盖层下发育的噬菌斑数量少约60%。兔肾细胞和人胚肺成纤维细胞比小鼠胚胎成纤维细胞对所有测试变体的感染更敏感。
Rapp, Fred (Baylor University College of Medicine, Houston, Tex.). Variants of herpes simplex virus: isolation, characterization, and factors influencing plaque formation. J. Bacteriol. 86:985–991. 1963.—Variants of herpes simplex virus were isolated which differed in plaque size and in virulence for rabbits and mice. Keratitis on the rabbit cornea and generalized disease were associated with the large, but not the small, plaque variants. The large plaque variants were about as neurovirulent as was the parent strain for weanling mice, but the majority of the small plaque variants were only 1% as virulent for mice as was the parent virus. Agar inhibited the formation of plaques by the parent strain and all variants tested; development of plaques was retarded and the number of plaques was approximately 60% less than those developing under an overlay in which methyl cellulose had been substituted for the agar. Rabbit kidney cells and human embryonic lung fibroblasts were more sensitive than mouse embryo fibrolasts to infection with all variants tested.