Simple peptides derived from the ribosomal core potentiate RNA polymerase ribozyme function
Simple peptides derived from the ribosomal core potentiate RNA polymerase ribozyme function
复制标题
DOI：
10.1038/nchem.2739
复制
发表时间：
2017-04-01
期刊：
影响因子：
21.8
通讯作者：
Holliger, Philipp
中科院分区：
文献类型：
--
作者：
Tagami, Shunsuke;Attwater, James;Holliger, Philipp
核酸和多肽之间功能相互作用的出现是生命起源的关键转变，并且仍然是所有生物学的核心。然而，简单的非编码肽如何以及为何对 RNA 功能变得至关重要尚不清楚。在这里，我们表明，来自两个核糖体亚基核心的假定的古老肽片段增强了RNA聚合酶核酶（RPR）的功能，就像包含赖氨酸或非蛋白赖氨酸类似物鸟氨酸或在较小程度上二氨基丁酸的衍生均聚肽一样，无论手性或手性纯度如何。赖氨酸十肽通过引物-模板对接促进全酶组装，从而增强 RPR 功能，加速 RPR 进化，并允许 RPR 催化的 RNA 在接近生理 (>= 1 mM) Mg2+ 浓度下合成，从而实现膜原细胞内的模板化 RNA 合成。我们的结果概述了组成简单的混合手性肽如何增强早期 RNA 的功能潜力并促进第一个原始细胞的出现。
The emergence of functional interactions between nucleic acids and polypeptides was a key transition in the origin of life and remains at the heart of all biology. However, how and why simple non-coded peptides could have become critical for RNA function is unclear. Here, we show that putative ancient peptide segments from the cores of both ribosomal subunits enhance RNA polymerase ribozyme (RPR) function, as do derived homopolymeric peptides comprising lysine or the nonproteinogenic lysine analogues ornithine or, to a lesser extent, diaminobutyric acid, irrespective of chirality or chiral purity. Lysine decapeptides enhance RPR function by promoting holoenzyme assembly through primer-template docking, accelerate RPR evolution, and allow RPR-catalysed RNA synthesis at near physiological (>= 1 mM) Mg2+ concentrations, enabling templated RNA synthesis within membranous protocells. Our results outline how compositionally simple, mixedchirality peptides may have augmented the functional potential of early RNAs and promoted the emergence of the first protocells.