EVIDENCE THAT PARTICULATE METHANE MONOOXYGENASE AND AMMONIA MONOOXYGENASE MAY BE EVOLUTIONARILY RELATED
EVIDENCE THAT PARTICULATE METHANE MONOOXYGENASE AND AMMONIA MONOOXYGENASE MAY BE EVOLUTIONARILY RELATED
复制标题
DOI：
10.1111/j.1574-6968.1995.tb07834.x
复制
发表时间：
1995-10-15
影响因子：
2.1
通讯作者：
MURRELL, JC
中科院分区：
文献类型：
--
作者：
HOLMES, AJ;COSTELLO, A;MURRELL, JC
编码颗粒甲烷单加氧酶和氨单加氧酶的基因具有高度的序列同一性。基于27-kDa多肽之间的共享氨基酸序列区域设计了简并寡核苷酸引物，该区域被认为含有颗粒甲烷单加氧酶和氨单加氧酶的活性位点。通过 PCR 扩增、克隆和测序来自多种甲烷氧化细菌和硝化细菌的编码这些多肽（pmoA 和 amoA）的基因的 525 bp 内部 DNA 片段。包括甲烷氧化菌（α-和γ-变形菌门）和氨氧化硝化细菌（β-和γ-变形菌门）的每个系统发育群的代表。对这些基因的预测氨基酸序列的分析揭示了一级和二级结构的强烈保守性。与 AmoA 序列相比，海洋亚硝化球菌 AmoA 与来自其他γ-变形菌门成员的 PmoA 序列具有更高的同一性。这些结果表明，颗粒甲烷单加氧酶和氨单加氧酶是进化相关的酶，尽管它们在这些细菌中具有不同的生理作用。
Genes encoding particulate methane monooxygenase and ammonia monooxygenase share high sequence identity. Degenerate oligonucleotide primers were designed, based on regions of shared amino acid sequence between the 27-kDa polypeptides, which are believed to contain the active sites, of particulate methane monooxygenase and ammonia monooxygenase. A 525-bp internal DNA fragment of the genes encoding these polypeptides (pmoA and amoA) from a variety of methanotrophic and nitrifying bacteria was amplified by PCR, cloned and sequenced. Representatives of each of the phylogenetic groups of both methanotrophs (alpha- and gamma-Proteobacteria) and ammonia-oxidizing nitrifying bacteria (beta-and gamma-Proteobacteria) were included, Analysis of the predicted amino acid sequences of these genes revealed strong conservation of both primary and secondary structure. Nitrosococcus oceanus AmoA showed higher identity to PmoA sequences from other members of the gamma-Proteobacteria than to AmoA sequences. These results suggest that the particulate methane monooxygenase and ammonia monooxygenase are evolutionarily related enzymes despite their different physiological roles in these bacteria.