Wound healing of different molecular weight of hyaluronan; in-vivo study
Wound healing of different molecular weight of hyaluronan; in-vivo study
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DOI：
10.1016/j.ijbiomac.2016.05.021
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发表时间：
2016-08-01
影响因子：
8.2
通讯作者：
Jancar, J.
中科院分区：
文献类型：
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作者：
Fouda, Moustafa M. G.;Abdel-Mohsen, A. M.;Jancar, J.
在受损的伤口愈合过程中，细胞和介质的募集被改变，从而延缓了这一过程。为了克服这一问题，研究了不同分子量透明质酸对老年大鼠伤口愈合的影响；（low-HA1）， （High-HA2）， （Medium-HA3）和银纳米颗粒（High-HA4）进行了研究。选择优质HA对糖尿病创面进行进一步研究。我们的结果指出，在老年大鼠伤口肉芽明显不足，这是伴随着损伤愈合过程。老年大鼠HA2或HA4处理后，肉芽和真皮结构均有改善。此外，HA2和HA4在伤口上的致病菌数量在前24 h内下降。ha4处理的老年大鼠伤口大小明显小于其他HA1、HA2或ha3处理的老年大鼠。此外，糖尿病还会降低糖尿病模型中炎症细胞因子的水平。相反，在糖尿病模型中，HA4被发现使炎症细胞因子水平正常化。此外，HA4还能恢复糖尿病模型中的所有氧化和毒性标志物。这一数据证实了HA4在老年和糖尿病大鼠伤口愈合中改善肉芽和炎症介质的关键作用。(C) 2016 Elsevier B.V.版权所有
Recruitment of cells and mediators is altered during impaired wound healing, thereby delaying this process. To overcome this problem, the correlation of wound healing in older rats, and the impact of different molecular weight of hyaluronan without silver nanoparticles; (low-HA1), (High-HA2), (Medium-HA3) and with silver nanoparticles (High-HA4) is investigated. The superior HA were selected to be further investigated onto diabetic wounds. Our results pointed to a marked deficiency in wounds granulation in older rats, which was accompanied with impairment of healing process. In older rats group treated with HA2 or HA4, granulation and dermal construction were improved. Furthermore, the number of pathogenic bacteria on wounds was declined throughout the first 24 h by HA2 and HA4. The wound size in HA4-treated older rats was significantly smaller than that in other HA1, HA2 or HA3-treated older ones. Also, diabetes impaired the level of inflammatory cytokine, in diabetic model. On contrary, HA4 was found to normalize the level of inflammatory cytokine, in the diabetic model. Furthermore, HA4 was found to recover all oxidative and toxicity markers in diabetic models. This data confirms the critical role of HA4 to improve granulation and inflammatory mediators in impaired older and diabetic rat wound healing. (C) 2016 Elsevier B.V. All rights reserved.