High-magnitude, virus-specific CD4 T-cell response in the central nervous system of coronavirus-infected mice
High-magnitude, virus-specific CD4 T-cell response in the central nervous system of coronavirus-infected mice
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DOI：
10.1128/jvi.75.6.3043-3047.2001
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发表时间：
2001-03-01
影响因子：
5.4
通讯作者：
Perlman, S
中科院分区：
文献类型：
--
作者：
Haring, JS;Pewe, LL;Perlman, S
The neurotropic JHM strain of mouse hepatitis virus (MHV) causes acute encephalitis and chronic demyelinating encephalomyelitis in rodents. Previous results indicated that CD8 T cells infiltrating the central nervous system (CNS) were largely antigen specific in both diseases. Herein we show that by 7 days postinoculation, nearly 30% of the CD4 T cells in the acutely infected CNS were MHV specific by using intracellular gamma interferon (IFN-gamma) staining assays. In mice with chronic demyelination, 10 to 15% of the CD4 T cells secreted IFN-gamma in response to MHV-specific peptides. Thus, these results show that infection of the CNS is characterized by a large influx of CD4 T cells specific for MHV and that these cells remain functional, as measured by cytokine secretion, in mice with chronic demyelination.
