HETEROGENEITY IN THE IMMUNOLOCALIZATION OF CYTOKERATIN SPECIFIC MONOCLONAL-ANTIBODIES IN THE RAT EYE - EVALUATION OF UNUSUAL EPITHELIAL TISSUE ENTITIES
HETEROGENEITY IN THE IMMUNOLOCALIZATION OF CYTOKERATIN SPECIFIC MONOCLONAL-ANTIBODIES IN THE RAT EYE - EVALUATION OF UNUSUAL EPITHELIAL TISSUE ENTITIES
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DOI：
10.1007/bf00266749
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
KASPER, M
中科院分区：
文献类型：
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作者：
KASPER, M
利用39种细胞角蛋白多肽特异性单克隆抗体和一种抗CK20多克隆抗血清，研究了细胞角蛋白和静脉蛋白在大鼠眼组织中的免疫细胞化学定位。视网膜和睫状体色素上皮仅表达细胞角蛋白8和18，而胎儿视网膜色素上皮和视网膜和睫状体过渡区的局部成人上皮表现出细胞角蛋白19的反应性。相比之下，非色素纤毛上皮仅呈波形蛋白阳性。用细胞角蛋白7、8、18和19特异性单克隆抗体对大鼠结膜内分布的杯状细胞簇进行选择性染色。其中染色杯状细胞的一组细胞角蛋白8和18特异性单克隆抗体，以及细胞角蛋白8和18阳性的内对照，对大鼠眼睛的细胞角蛋白8和18阳性的神经外表皮细胞和大鼠脉络膜丛上皮均无反应。这表明了神经外胚层来源的角蛋白和其他细胞角蛋白在表型上的差异，例如构象表位的改变。角膜和结膜上皮中鳞状上皮型细胞角蛋白的分布更为复杂。边缘区作为连接两个上皮的过渡区，表现出细胞角蛋白模式的变化。总的来说，该研究强调了扩大抗体小组的必要性，以避免在细胞角蛋白的免疫定位中产生误导性结果。
The immunocytochemical localization of cytokeratin and vimentin in rat eye tissues was investigated using a panel of 39 monoclonal antibodies specific for single or multiple of cytokeratin polypeptides and one polyclonal anti CK20 antiserum. The retinal and the ciliary body pigment epithelia only expressed cytokeratins 8 and 18, whereas the fetal retinal pigment epithelium and focally the adult epithelium, in the transition zone of retina and ciliary body, exhibited a reactivity for cytokeratin 19. In contrast, the non-pigmented ciliary epithelium was positive for vimentin only.In the rat conjunctiva distributed goblet cell clusters were selectively stained with cytokeratin 7, 8, 18 and 19 specific monoclonal antibodies. Among them a group of cytokeratin 8 and 18 specific monoclonal antibodies which stained the goblet cells as well as cytokeratin 8 and 18 positive internal controls did not react with either the cytokeratin 8 and 18 positive neuroectodermal cells of the rat eye nor the rat choroid plexus epithelium. This indicates differences in the phenotype e.g. conformational epitope changes, of neuroectodermal derived and other cytokeratins. The corneal and conjunctival epithelium showed a more complex distribution of squamous epithelium type cytokeratins. The limbal region as a transient zone connecting both epithelia exhibited a changing cytokeratin pattern. In general, the study emphasized the necessity to work with an enlarged antibody panel to avoid misleading results in the immunolocalization of cytokeratins.