Potential effects of the combination of nicotinamide, vitamin B2 and vitamin C on oxidative-mediated hepatotoxicity induced by thioacetamide.
Potential effects of the combination of nicotinamide, vitamin B2 and vitamin C on oxidative-mediated hepatotoxicity induced by thioacetamide.
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DOI：
10.1186/s12944-018-0674-z
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发表时间：
2018-02-14
影响因子：
4.5
通讯作者：
Al Tamimi J
中科院分区：
文献类型：
--
作者：
Bashandy SAE;Ebaid H;Abdelmottaleb Moussa SA;Alhazza IM;Hassan I;Alaamer A;Al Tamimi J
肝病是埃及最重要的传统公共卫生问题之一。氧化应激是由于这种病理条件，进一步有助于肝损伤的开始和进展。在本研究中，我们研究了烟酰胺（NA）、维生素B2（VB 2）和维生素C（VC）的强大抗氧化能力是否可以改善TAA诱导的氧化应激介导的大鼠肝损伤。将36只白化病大鼠随机分为6组：对照组、TAA组（IP注射TAA，剂量为200 mg/Kg，每周三次，持续两个月）; TAA + NA组（与对照组相同，除TAA外，每天给予NA 200 mg/kg）; TAA + VB 2组TAA + VC组（在注射TAA的同时沿着给予维生素C 200 mg/kg/d）。TAA + NA + VB + VC组（大鼠在预先注射上述相应剂量的TAA中每日给予三种维生素）。用TAA处理大鼠导致血清样品中天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）、总胆红素、胆固醇、甘油三酯、低密度脂蛋白（LDL）和肿瘤坏死因子-α（TNF-α）显著升高。此外，丙二醛（MDA），羟脯氨酸和一氧化氮（NO）也显着增加，而还原型谷胱甘肽（GSH），超氧化物歧化酶（SOD）和过氧化氢酶（CAT）在TAA处理的大鼠肝样品中显着受损。大鼠给予NA，VB 2和VC作为单独或组合改善TAA的有害作用，这是由组织病理学证实。然而，三种维生素的组合被发现比每种维生素更有效。我们的工作表明，NA，VB 2和VC相互串扰，作为一个更有效的生物化学链的抗氧化防御TAA诱导的毒性在体内。
The liver disease is one of the most important traditional public health problems in Egypt. Oxidative stress is attributed to such pathological condition that further contributes to the initiation and progression of liver injury. In the present study, we have investigated if the strong antioxidant power of Nicotinamide (NA), Vitamin B2 (VB2), and Vitamin C (VC) can ameliorate TAA-induced oxidative stress-mediated liver injury in the rats. Thirty-six albino rats were divided into six groups: Control group; TAA group (IP injection with TAA at a dosage of 200 mg/Kg three times a week for two months); TAA + NA group (rats administered with NA at a dosage of 200 mg/kg daily besides TAA as in the control); TAA + VB2 group (rats administered with vitamin B2 at a dosage of 30 mg/kg daily besides injection with TAA); TAA + VC group (rats administered with vitamin C at a dosage of 200 mg/kg daily along with injection of TAA). TAA + NA + VB + VC group (rats administered the with the three vitamins daily in TAA pre-injected at the respective doses described above). Treatment of rats with TAA led to a significant elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total bilirubin, cholesterol, triglycerides, low-density lipoprotein (LDL) and tumor necrosis factor-alpha (TNF-α) in the serum samples. Moreover, malondialdehyde (MDA), hydroxyproline and nitic oxide (NO) were also significantly increased in the TAA-treated rats, while reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were significantly compromised in the hepatic samples. Rats administered with NA, VB2, and VC as individually or in combination ameliorated the deleterious effects of TAA that was confirmed by histopathology. However, the combination of the three vitamins was found more effective as compared to each of the vitamins. Our work demonstrates that NA, VB2, and VC cross-talk with each other that act as a more potent biochemical chain of antioxidant defense against TAA-induced toxicities in vivo.
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影响因子：
3.6
作者：
Godin, Adriana M.;Ferreira, Wallace C.;Coelho, Marcio M.
通讯作者：
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影响因子：
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DOI：
10.1016/j.ijbiomac.2016.05.021
发表时间：
2016-08-01
影响因子：
8.2
作者：
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作者：
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