Mechanical properties and in vitro biocompatibility of porous zein scaffolds
Mechanical properties and in vitro biocompatibility of porous zein scaffolds
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DOI：
10.1016/j.biomaterials.2006.02.019
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发表时间：
2006-07-01
期刊：
影响因子：
14
通讯作者：
Wang, JY
中科院分区：
文献类型：
--
作者：
Gong, SJ;Wang, HJ;Wang, JY
采用盐浸法制备了利用疏水性蛋白玉米醇溶蛋白的多孔支架，用于组织工程。支架总孔隙率为75.3-79.0%，压缩杨氏模量为(28.2±6.7)MPa-(86.6±19.9)MPa，抗压强度为(2.5±1.2)MPa-(11.8±1.7)MPa，14小时内胶原酶降解率为36%，胃蛋白酶降解率为89%。体外培养天数。通过扫描电子显微镜（SEM），位于多孔支架表面和内部的孔形态显示出良好的孔互连性。大鼠间充质干细胞（MSC）可以在多孔玉米醇溶蛋白支架上粘附、生长、增殖并分化为成骨细胞。在地塞米松作用下，细胞表现出比没有地塞米松的MSCs相对更高的碱性磷酸酶（ALP）活性和更高的增殖活性（p < 0.05）。 (c) 2006 Elsevier Ltd. 保留所有权利。
A porous scaffold utilizing hydrophobic protein zein was prepared by the salt-leaching method for tissue engineering. The scaffolds possessed a total porosity of 75.3-79.0%, compressive Young's modulus of (28.2 +/- 6.7)MPa-(86.6 +/- 19.9)MPa and compressive strength of (2.5 +/- 1.2)MPa-(11.8 +/- 1.7)MPa, the percentage degradation of 36% using collagenase and 89% using pepsin during 14 days incubation in vitro. The morphology of pores located on the surface and within the porous scaffolds showed good pore interconnectivity by scanning electron microscopy (SEM). Rat mesebchymal stern cells (MSCs) could adhere, grow, proliferate and differentiate toward osteoblasts on porous zein scaffold. With the action of dexamethasone, the cells showed a relative higher activity of alkaline phosphatase (ALP) and a higher proliferating activity (p < 0.05) than those of MSCs without dexamethasone. (c) 2006 Elsevier Ltd. All rights reserved.