The BALB/c mouse: Effect of standard vivarium lighting on retinal pathology during aging
The BALB/c mouse: Effect of standard vivarium lighting on retinal pathology during aging
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DOI：
10.1016/j.exer.2015.04.009
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发表时间：
2015-06-01
影响因子：
3.4
通讯作者：
Hollyfield, Joe G.
中科院分区：
文献类型：
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作者：
Bell, Brent A.;Kaul, Charles;Hollyfield, Joe G.
在正常的室内光照条件下饲养的BALB/cJ小鼠可以表现出严重的视网膜异常，包括视网膜褶皱、自身荧光炎症细胞和光感受器变性。为了探讨衰老过程中外视网膜对循环光照的敏感性，我们采用扫描激光眼科镜（SLO）、光谱域光学相干断层扫描（OCT）和常规组织病理学方法对BALB/cJ小鼠进行了研究。小鼠饲养于低照度（提取液/笼内：13 lx/1 lx）的循环光照下（光照14 h：暗10 h）。视网膜成像（大约在出生后第70天）用于筛查任何先前存在的异常并建立基线。将视网膜正常的小鼠分为A、B、C组，分别置于笼子照明的笼架底部（A、B组）和顶部（C组）
BALB/cJ mice housed under normal vivarium lighting conditions can exhibit profound retinal abnormalities, including retinal infoldings, autofluorescent inflammatory cells, and photoreceptor degeneration. To explore the sensitivity of the outer retina to cyclic lighting during aging, a cohort of BALB/cJ mice was evaluated with Scanning Laser Ophthalmoscopy (SLO), Spectral-Domain Optical Coherence Tomography (OCT) and conventional histopathology. Mice were bred and reared in a low-illuminance (extracage/intracage: 13 lx/1 lx) vivarium under cyclic light (14 h light: 10 h dark). Retinal imaging (around postnatal day 70) was performed to screen for any pre-existing abnormalities and to establish a baseline. Mice with normal retinas were separated into groups (A, B, C) and placed on bottom (Groups A & B) or top (Group C) of the cage racks where cage illumination was