Anatase Nanosurface Regulates MicroRNAs
Anatase Nanosurface Regulates MicroRNAs
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DOI：
10.1097/scs.0b013e3181534ab3
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发表时间：
2008-03
影响因子：
0.9
通讯作者：
A. Palmieri;F. Pezzetti;G. Brunelli;Marzia Arlotti;L. Lo Muzio;A. Scarano;C. Rubini;V. Sollazzo;L. Massari;F. Carinci
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文献类型：
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作者：
A. Palmieri;F. Pezzetti;G. Brunelli;Marzia Arlotti;L. Lo Muzio;A. Scarano;C. Rubini;V. Sollazzo;L. Massari;F. Carinci
钛因其良好的机械和化学性能而成为假体器械所用材料的标准。当暴露于氧气时，钛变成具有生物相容性并能够诱导骨整合的氧化物。二氧化钛有3种同素异形体：板钛矿、金红石和金红石。锐钛矿可以制备成胶体悬浮液，然后用于涂覆表面。锐钛矿涂层（AC）可能具有特定的生物学效应，并特异性诱导骨形成。为了获得更多关于AC成骨作用的信息，我们使用microRNA（miRNA）微阵列技术来研究暴露于AC的成骨细胞的翻译调控。转导、转录和翻译是细胞活性调节的3个水平。最近，一种新型的翻译调控被确定：RNA干扰。RNA干扰是一个过程，其中miRNA（即，19-23个核苷酸的非编码RNA）可以诱导序列特异性mRNA降解和/或翻译抑制。人类基因组编码几百种可以转录后抑制数千个基因的miRNA。miRNA寡核苷酸芯片提供了一种在人类样本中进行全基因组miRNA谱分析的新方法。通过使用包含329个探针的miRNA微阵列，我们在AC培养的成骨样细胞系（MG-63）中鉴定了几种表达显著改变的miRNA。据我们所知，报告的数据构成了第一个研究成骨细胞暴露于AC的翻译调控。它们可以更好地理解骨再生的分子机制，并作为比较具有相似临床效果的其他材料的模型。
Titanium is the criterion standard among materials used for prosthetic devices because of its good mechanical and chemical properties. When exposed to oxygen, titanium becomes an oxide that is biocompatible and able to induce osseointegration. There are 3 allotropic forms of titanium dioxide: brookite, rutile, and anatase. Anatase can be prepared as a colloidal suspension and then used to coat surfaces. Anatase coating (AC) can potentially have specific biologic effects and specifically induce bone formation. To get more information as regards the osteogenic effect of AC, we used microRNA (miRNA) microarray techniques to investigate translation regulation in osteoblasts exposed to AC. Transduction, transcription, and translation are the 3 levels of regulation of cell activity. Recently, a new type of translation regulation has been identified: RNA interference. RNA interference is a process in which miRNA (i.e., noncoding RNAs of 19-23 nucleotides) can induce sequence-specific mRNA degradation and/or translational repression. The human genome encodes a few hundred miRNAs that can posttranscriptionally repress thousands of genes. miRNA oligonucleotide microarray provides a novel method of carrying out genome-wide miRNA profiling in human samples. By using miRNA microarrays containing 329 probes designed from human miRNA sequences, we identified in osteoblast-like cell line (MG-63) cultured with AC several miRNA whose expression had been significantly modified. The data reported constitute, to our knowledge, the first study on translation regulation in osteoblasts exposed to AC. They can be relevant to a better understanding of the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.