Dynamic sieving capillary electrophoresis analysis of xylitol selenite-induced apoptosis in SMMC-7221 cells
Dynamic sieving capillary electrophoresis analysis of xylitol selenite-induced apoptosis in SMMC-7221 cells
复制标题
DOI：
10.1007/s10529-012-0945-0
复制
发表时间：
2012-09-01
影响因子：
2.7
通讯作者：
Qi, Chao
中科院分区：
文献类型：
--
作者：
Lei, Ming;Chen, Didi;Qi, Chao
DNA阶梯片段被认为是细胞凋亡的生化标志，用毛细管电泳技术快速、成功地分离了DNA阶梯片段。首次测定了木糖醇亚硒酸酯诱导的核小体间DNA片段，并以羟丙基甲基纤维素（HPMC）为筛分基质，进行了动态筛分毛细管电泳。建立了校正曲线（r(2) = 0.991），在木糖醇亚硒酸盐存在下形成了两种不同核小体（140 bp和180 bp）的倍数。硒化合物通过增加细胞凋亡抑制动物模型、SMMC-7221细胞和其他几种细胞的癌变。该方法对木糖醇亚硒酸酯和其他硒化合物的抗癌活性有一定的研究价值，且对小片段的检测比平板凝胶电泳更有效。
DNA ladder fragments, regarded as a biochemical hallmark of apoptosis, have been separated quickly and successfully by capillary electrophoresis. Inter-nucleosomal DNA fragmentations induced by xylitol selenite were determined for the first time, while hydroxypropylmethylcellulose (HPMC) was served as the sieving matrix in dynamic sieving capillary electrophoresis. The calibration curve (r(2) = 0.991) was established and multiples of two different nucleosomes (140 and 180 bp) were formed in the presence of xylitol selenite. Selenium compounds inhibited carcinogenesis in animal models, SMMC-7221 cells and several other cells by increasing apoptosis. The described method was useful in elucidating the anticancer activities of xylitol selenite and other selenium compounds, which was more effective to detect small fragments than slab gel electrophoresis.