An immunohistochemical observations on the oviduct of the goat.
An immunohistochemical observations on the oviduct of the goat.
复制标题
山羊输卵管的免疫组织化学观察。
DOI：
10.1111/rda.12349
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
M. Maretta
中科院分区：
文献类型：
--
作者：
E. Marettová;M. Maretta
输卵管在调节配子运输和受精中起着重要作用。在这些功能中起主要作用的有平滑肌细胞和输卵管内壁的纤毛上皮。所有的功能都受荷尔蒙和神经系统的影响。本研究的目的是免疫组织化学检查以下结构：衬里上皮，平滑肌细胞，弹性纤维和神经纤维。为此，使用了以下抗体：细胞角蛋白18、S-100蛋白、乙酰化α-微管蛋白、平滑肌肌动蛋白、desmin和弹性蛋白。细胞角蛋白18和S-100表达阳性。纤毛及纤毛细胞基底体相关结构乙酰化α-微管蛋白阳性。粘膜和肌层平滑肌细胞α-平滑肌肌动蛋白（SMA）和desmin阳性。高密度的神经纤维与乙酰化α-微管蛋白和S100蛋白在粘膜、肌层和浆膜中存在正反应。弹性蛋白阳性的弹性纤维在粘膜褶皱的底部和肌肉层形成致密的网络。这些纤维的密集网络伴随着血管。据推测，它们与平滑肌细胞一起参与卵子的运输和血流调节。
The oviduct has an important role in regulating transport of gametes and fertilization. The main role in these functions has a smooth muscle cells and ciliated epithelium lining the oviduct. All functions are under the influence of hormonal and nervous system. The objective of this study was immunohistochemically to examine the following structures: lining epithelium, smooth muscle cells, elastic fibres and nerve fibres. For this purpose, the following antibodies were used: cytokeratin 18, S-100 protein, acetylated α-tubulin, smooth muscle actin, desmin and elastin. Ciliary and secretory cells of the lining epithelium were positive for cytokeratin 18 and S-100 protein. Cilia and the basal body-associated structures of ciliary cells were positive to acetylated α-tubulin. Smooth muscle cells (SMC) in mucosa and of the muscular layer were positive for α-smooth muscle actin (SMA) and desmin. High density of nerve fibres positively reacted to acetylated α-tubulin and S100 protein was present in the mucosa, muscular layer and serosa. Elastic fibres positive for elastin form a dense network at the base of the mucosal folds and in the muscle layer. A dense network of these fibres is accompanying the blood vessels. It is supposed that together with smooth muscle cells they are involved in the transport of ovum and in blood flow regulation.
DOI：
10.1530/rep-12-0279
发表时间：
2012-12
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Coy P;García-Vázquez FA;Visconti PE;Avilés M
通讯作者：
Avilés M