Analysis of hepatic oxidative stress status by electron spin resonance spectroscopy and imaging
Analysis of hepatic oxidative stress status by electron spin resonance spectroscopy and imaging
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DOI：
10.1016/s0891-5849(99)00280-4
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发表时间：
2000-03-15
影响因子：
7.4
通讯作者：
Kamada, H
中科院分区：
文献类型：
--
作者：
Togashi, H;Shinzawa, H;Kamada, H
实时检测体内产生的自由基可能有助于阐明自由基在疾病过程中的病理生理作用。在可用于研究生物系统中自由基生成的技术中，电子自旋共振（ESR）已成为检测和鉴定的有力工具。本文首先回顾了利用X-带ESR波谱技术对氧自由基的自旋捕获检测，然后介绍了利用自旋捕获5，5-二甲基-1-吡咯啉-N-氧化物和ESR波谱技术对离体大鼠肝脏缺血/再灌注液中超氧自由基和羟基自由基的检测。本文还综述了ESR在体内自由基检测和外源性自由基体内成像的现状。此外，我们表明，在体内ESR计算机断层扫描与3-氨基甲酰基-2，2，5，5-四甲基吡咯烷-1-氧基可能是有用的非侵入性解剖成像，也为成像的肝脏氧化应激在体内。(C)2000 Elsevier Science Inc.
Real-time detection of free radicals generated within the body may contribute to clarify the pathophysiological role of free radicals in disease processes. Of the techniques available for studying the generation of free radicals in biological systems, electron spin resonance (ESR) has emerged as a powerful tool for detection and identification. This article begins with a review of spin trapping detection of oxygen-centered radicals using X-band ESR spectroscopy and then describes the detection of superoxide and hydroxyl radicals by the spin trap 5,5-dimethyl-1-pyrroline-N-oxide and ESR spectroscopy in the perfusate from isolated perfused rat livers subjected to ischemia/reperfusion. This article also reviews the current status of ESR for the in vivo detection of free radicals and in vivo imaging of exogenously administered free radicals. Moreover, we show that in vivo ESR-computed tomography with 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl may be useful for noninvasive anatomical imaging and also for imaging of hepatic oxidative stress in vivo. (C) 2000 Elsevier Science Inc.