A spectrophotometric analysis of pigments in apples
A spectrophotometric analysis of pigments in apples
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DOI：
10.1023/a:1016780624280
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发表时间：
2001-09-01
影响因子：
1.4
通讯作者：
Reshetnikova, IV
中科院分区：
文献类型：
--
作者：
Solovchenko, AE;Chivkunova, OB;Reshetnikova, IV
采用传统的极性有机溶剂（丙酮或甲醇）的色素提取方法进行了比较，那些采用氯仿-甲醇混合物。我们发现，分光光度法分析苹果皮中的色素，必须从样品中预先提取的表皮脂质与氯仿和MgO必须加入均质过程中，以防止色素降解。传统的提取方法不能完全提取色素，提取物中含有大量的吸光杂质，采用氯仿-甲醇萃取法可以显著降低吸光杂质的含量，提高叶绿素和类胡萝卜素含量测定的准确度和灵敏度。此外，这种提取方法被证明是有用的酚类物质（花青素和黄酮类化合物）的水-甲醇馏分的提取物的分析。
Methods of pigment extraction using traditional polar organic solvents (acetone or methanol) were compared to those employing a chloroform-methanol mixture. We found that, for spectrophotometric pigment analysis in the apple peel, the cuticular lipids must be preliminarily extracted from the samples with chloroform and MgO must be added during homogenization to prevent pigment degradation. The traditional extraction did not result in the complete extraction of intact pigments, and such extracts contained a considerable amount of light-absorbing impurities, The application of chloroform-methanol extraction allowed us to markedly reduce the content of such impurities and to increase the accuracy and sensitivity of the measurement of the content of chlorophylls and carotenoids. In addition, this extraction method proved useful for the analysis of phenolic substances (anthocyanins and flavonoids) in the water-methanol fraction of the extracts.