Porphyromonas gingivalis lipopolysaccharide lipid A heterogeneity differentially modulates the expression of IL-6 and IL-8 in human gingival fibroblasts
Porphyromonas gingivalis lipopolysaccharide lipid A heterogeneity differentially modulates the expression of IL-6 and IL-8 in human gingival fibroblasts
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DOI：
10.1111/j.1600-051x.2011.01741.x
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发表时间：
2011-08-01
影响因子：
6.7
通讯作者：
Jin, Lijian
中科院分区：
文献类型：
--
作者：
Herath, Thanuja D. K.;Wang, Yu;Jin, Lijian
目的：牙龈卟啉单胞菌脂多糖（LPS）显示出显著的结构异质性，包含四酰化（LPS 1435/1449）和五酰化（LPS 1690）脂质A结构。本研究调查了牙龈卟啉单胞菌LPS的两种亚型对人牙龈成纤维细胞（HGFs）中IL-6、IL-8和TNF-α表达的影响。材料和方法：在剂量（1 ng-10 μ g/ml）和时间依赖性（2 - 48 h）实验中，用牙龈卟啉单胞菌LPS 1435/1449和LPS 1690刺激HGFs。提取总RNA和蛋白质，并分别通过定量实时聚合酶链反应和酶联免疫吸附测定用于分析IL-6、IL-8和TNF-α转录物以及IL-6和IL-8蛋白。牙龈卟啉单胞菌LPS 1690显著上调IL-6和IL-8的mRNA和蛋白表达，而牙龈卟啉单胞菌LPS 1435/1449不诱导显著的宿主应答。牙龈卟啉单胞菌LPS 1690上调的IL-6和IL-8的表达水平随时间的推移而持续增加。与此相反，TNF-α的转录表达迅速上调牙龈卟啉单胞菌LPS 1690刺激后2 h后，并逐渐下降afters.Conclusions：这项研究表明，牙龈卟啉单胞菌LPS异质性可能差异调节促炎细胞因子在HGF的表达，这可能有助于牙周病的发病机制。
Aim: Porphyromonas gingivalis lipopolysaccharide (LPS) displays a significant amount of structural heterogeneity, containing both tetra-(LPS1435/1449) and penta-acylated (LPS1690) lipid A structures. This study investigated the effects of the two isoforms of P. gingivalis LPS on the expression of IL-6, IL-8 and TNF-alpha in human gingival fibroblasts (HGFs).Materials and methods: HGFs were stimulated with P. gingivalis LPS1435/1449 and LPS1690 in both dose-(1 ng-10 mu g/ml) and time-dependent (2-48 h) experiments. Total RNA and protein were extracted and used for analysis of the IL-6, IL-8 and TNF-a transcripts as well as IL-6 and IL-8 proteins, by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.Results: P. gingivalis LPS1690 significantly up-regulated the mRNA and protein expression of IL-6 and IL-8, whereas P. gingivalis LPS1435/1449 did not induce significant host response. The expression levels of IL-6 and IL-8 up-regulated by P. gingivalis LPS1690 continuously increased with time course. In contrast, TNF-alpha transcript expression was up-regulated promptly by P. gingivalis LPS1690 after 2 h of stimulation and gradually declined afterwards.Conclusions: This study suggests that P. gingivalis LPS heterogeneity may differentially modulate the pro-inflammatory cytokine expression in HGFs, which may contribute to periodontal pathogenesis.