Transcription factor-DNA binding: beyond binding site motifs.
Transcription factor-DNA binding: beyond binding site motifs.
复制标题
DOI：
10.1016/j.gde.2017.02.007
复制
发表时间：
2017-04
影响因子：
4
通讯作者：
Bulyk ML
中科院分区：
文献类型：
--
作者：
Inukai S;Kock KH;Bulyk ML
序列特异性转录因子（TF）通过与启动子和增强子DNA中的顺式调控元件结合来调节基因表达。虽然TF-DNA结合的研究集中在TF对一级核苷酸序列基序的内在偏好上，但最近的研究已经阐明了调节TF-DNA结合的额外复杂性层。在这篇综述中，我们讨论了识别TF结合偏好的技术发展，并强调了最近的发现，阐述了TF相互作用，局部DNA结构和基因组特征如何影响TF-DNA结合。我们强调新的方法来表征功能结合位点的基序，承诺通知我们的理解如何TF结合控制基因表达，并最终有助于表型。
Sequence-specific transcription factors (TFs) regulate gene expression by binding to cis-regulatory elements in promoter and enhancer DNA. While studies of TF–DNA binding have focused on TFs' intrinsic preferences for primary nucleotide sequence motifs, recent studies have elucidated additional layers of complexity that modulate TF–DNA binding. In this review, we discuss technological developments for identifying TF binding preferences and highlight recent discoveries that elaborate how TF interactions, local DNA structure, and genomic features influence TF–DNA binding. We highlight novel approaches for characterizing functional binding site motifs that promise to inform our understanding of how TF binding controls gene expression and ultimately contributes to phenotype.