Genome-wide association mapping identifies HvNIP2;2/HvLsi6 accounting for efficient boron transport in barley
Genome-wide association mapping identifies HvNIP2;2/HvLsi6 accounting for efficient boron transport in barley
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DOI：
10.1111/ppl.13340
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发表时间：
2021-02-09
影响因子：
6.4
通讯作者：
Bienert, Gerd Patrick
中科院分区：
文献类型：
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作者：
Jia, Zhongtao;Bienert, Manuela Desiree;Bienert, Gerd Patrick
硼（B）是植物生长所必需的矿质元素，作物种子B库对幼苗在低B土壤上生长至关重要。迄今为止，人们对B在谷类作物中的积累是如何受遗传控制的知之甚少。在这里，我们评估了春大麦（Hordeum vulgare L.）籽粒中B浓度的基因型变异。代表广泛遗传多样性的关联面板。利用全基因组关联分析方法，共检测到23个数量性状位点（QTL），发现籽粒B含量存在较大的遗传变异。HvNIP2; 2/HvLsi 6编码一种潜在的硼转运膜蛋白，与一个占籽粒B变异比例最大的主效QTL紧密连锁。基于使用异源表达系统和基因表达分析的运输研究，我们证明，HvNIP 2;2/HvLsi 6代表一个功能性B通道，其转录水平的表达变化与根和芽B浓度以及在B缺乏条件下根干物质的形成。
Boron (B) is an essential mineral element for plant growth, and the seed B pool of crops can be crucial when seedlings need to establish on low-B soils. To date, it is poorly understood how B accumulation in grain crops is genetically controlled. Here, we assessed the genotypic variation of the B concentration in grains of a spring barley (Hordeum vulgare L.) association panel that represents broad genetic diversity. We found a large genetic variation of the grain B concentration and detected in total 23 quantitative trait loci (QTLs) using genome-wide association mapping. HvNIP2;2/HvLsi6, encoding a potential B-transporting membrane protein, mapped closely to a major-effect QTL accounting for the largest proportion of grain B variation. Based on transport studies using heterologous expression systems and gene expression analysis, we demonstrate that HvNIP2;2/HvLsi6 represents a functional B channel and that expression variation in its transcript level associates with root and shoot B concentrations as well as with root dry mass formation under B-deficient conditions.