Delayed hypersensitivity to nonviable Mycoplasma pulmonis in mice is enhanced by dextran sulfate
Delayed hypersensitivity to nonviable Mycoplasma pulmonis in mice is enhanced by dextran sulfate
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硫酸葡聚糖增强小鼠对非活肺支原体的迟发性超敏反应
DOI：
10.1128/iai.39.2.823-829.1983
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发表时间：
1983
影响因子：
3.1
通讯作者：
M. Nakagawa
中科院分区：
文献类型：
--
作者：
M. Kishima;C. Kuniyasu;M. Nakagawa
采用小鼠足底迟发性肿胀模型评价硫酸葡聚糖(DS)对小鼠迟发型超敏反应的影响。7天后，用不活的肺支原体和DS皮下注射相同抗原的小鼠的足垫迟发性肿胀显著增加。在仅用无活性肺支原体或DS预处理后再用无活性肺支原体足垫注射的小鼠中，未检测到延迟性足垫肿胀。与静脉或腹膜腔内预处理相比，皮下预处理无存活的肺支原体和DS患者在引起延迟性足垫肿胀方面效果更好。在给小鼠注射肺炎支原体、鸡败血支原体、支原体或牛分枝杆菌后的第7天，小鼠的足底迟发性肿胀并未形成。在肺支原体和DS预处理后第3天，在足垫内注射不存活的肺支原体，没有引起明显的足垫肿胀，但在第7、14、21和28天在足垫注射导致显著的足垫肿胀。通过静脉注射小鼠脾细胞将迟发性足垫肿胀转移到未治疗的受体小鼠体内。静脉注射抗小鼠胸腺细胞兔血清给预先接种灭活肺分支杆菌和DS的小鼠，可显着减轻小鼠迟发性足肿胀。
The enhancing effect of dextran sulfate (DS) on delayed hypersensitivity to nonviable Mycoplasma pulmonis in mice was evaluated by means of delayed footpad swelling. Mice pretreated subcutaneously with nonviable M. pulmonis and DS had significantly increased delayed footpad swelling when the mice were injected subcutaneously in the footpad with the same antigen 7 days later. Delayed footpad swelling was not detected in mice pretreated only with nonviable M. pulmonis or DS followed by footpad injection with nonviable M. pulmonis. Subcutaneous pretreatment in the scruff of the neck with nonviable M. pulmonis and DS was more effective in eliciting delayed footpad swelling than intravenous or intraperitoneal pretreatment. Delayed footpad swelling did not develop when mice were injected in the footpad with nonviable Mycoplasma hyopneumoniae, M. gallisepticum, M. dispar, or M. bovis on day 7 after pretreatment with M. pulmonis and DS. Injection of nonviable M. pulmonis in the footpad on day 3 after pretreatment with M. pulmonis and DS resulted in no significant footpad swelling, but injection in the footpad on days 7, 14, 21, and 28 resulted in significant footpad swelling. Delayed footpad swelling was transferred by intravenous injection with spleen cells of mice which were pretreated with nonviable M. pulmonis and DS into nontreated recipient mice. Intravenous injection with anti-mouse thymus cell rabbit serum into the mice which were previously pretreated with nonviable M. pulmonis and DS reduced the delayed footpad swelling significantly.