Biomedical and biotechnology applications of noncontact femtosecond laser microsurgery of living cells
Biomedical and biotechnology applications of noncontact femtosecond laser microsurgery of living cells
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非接触式飞秒激光活细胞显微手术的生物医学和生物技术应用
DOI：
10.1063/1.4739909
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
M. Semenova
中科院分区：
文献类型：
--
作者：
I. Ilina;M. M. Rakityanskiy;D. Sitnikov;A. Ovchinnikov;M. Agranat;Y. Khramova;M. Semenova
我们采用飞秒 (fs) 激光脉冲分别解决重要的生物医学和生物技术问题、胚胎活检和细胞融合。我们报告了早期哺乳动物胚胎的完全非接触式激光介导的极体（PB）和滋养外胚层（TE）活检的结果。在前一种情况下，首先使用飞秒激光手术刀（Cr：镁橄榄石种子振荡器和再生放大器，100飞秒，10赫兹）在胚胎的外壳上钻一个开口，然后通过光镊子（连续光纤激光器，1064 nm）将PB从受精卵中取出。在后一种情况下，使用激光手术刀解剖孵化期间刚刚离开透明带 (ZP) 的 5-7 个 TE 细胞。激光脉冲的能量经过彻底优化，可防止细胞损伤并提供处理细胞的高活力。形态学和荧光分析表明，基于飞秒激光的胚胎活检不会影响进一步的体外胚胎发育。 We also demonstrated the possibilities of ...
We employed femtosecond (fs) laser pulses to solve important biomedical and biotechnology problems, embryo biopsy and cell fusion respectively. We report on the results of fully contactless laser-mediated polar body (PB) and trophectoderm (TE) biopsy of early mammalian embryos. In the former case the fs laser scalpel (Cr:Forsterite seed oscillator and a regenerative amplifier, 100 fs, 10 Hz) was initially used to drill an opening in the outer covering of the embryo, and then the PB was extracted out of the zygote by means of optical tweezers (cw fiber laser, 1064 nm). In the latter case the laser scalpel was employed to dissect 5-7 TE cells that had just left the zona pellucida (ZP) during the hatching. The energy of laser pulses was thoroughly optimized to prevent cell damage and provide high viability of treated cells. Morphological and fluorescent analysis showed that femtosecond laser-based embryo biopsy did not compromise further in vitro embryo development. We also demonstrated the possibilities of ...