Cytochrome c-d regulates developmental apoptosis in the Drosophila retina
Cytochrome c-d regulates developmental apoptosis in the Drosophila retina
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DOI：
10.1038/sj.embor.7400773
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发表时间：
2006-09-01
期刊：
影响因子：
7.7
通讯作者：
Mollereau, Bertrand
中科院分区：
文献类型：
--
作者：
Mendes, Cesar S.;Arama, Eli;Mollereau, Bertrand
细胞色素c （Cyt c）在半胱天冬酶激活中的作用已经从哺乳动物细胞培养研究中得到了很大程度的证实，但其在原位的生理相关性仍有待进一步研究。Cyt - c在无脊椎动物中的作用一直受到相当大的争议。果蝇基因组包含不同的细胞c基因：细胞c-p和细胞c-d。由于线粒体呼吸需要cyt c- p基因，因此cyt c- p功能的丧失会导致胚胎死亡。相比之下，cyt c- d突变体可以存活，但雄性不育。在这里，我们发现cyt c- d调节蛹眼的发育凋亡。Cyt c- d突变视网膜显示多余的间质细胞和周间质细胞的凋亡明显延迟。此外，凋亡蛋白酶激活因子1 （Ark）和caspase 9 （Dronc）的果蝇同源物在突变的视网膜组织中没有发生凋亡。此外，我们发现cyt c- d -与ark和drong一样-调节鸡毛数量，已知这取决于半胱天冬酶活性。总之，我们的结果表明，Cyt c在果蝇体细胞的半胱天冬酶调节中的作用。
The role of cytochrome c ( Cyt c) in caspase activation has largely been established from mammalian cell- culture studies, but much remains to be learned about its physiological relevance in situ. The role of Cyt c in invertebrates has been subject to considerable controversy. The Drosophila genome contains distinct cyt c genes: cyt c-p and cyt c-d. Loss of cyt c- p function causes embryonic lethality owing to a requirement of the gene for mitochondrial respiration. By contrast, cyt c- d mutants are viable but male sterile. Here, we show that cyt c- d regulates developmental apoptosis in the pupal eye. cyt c- d mutant retinas show a profound delay in the apoptosis of superfluous interommatidial cells and perimeter ommatidial cells. Furthermore, there is no apoptosis in mutant retinal tissues for the Drosophila homologues of apoptotic protease- activating factor 1 ( Ark) and caspase 9 ( Dronc). In addition, we found that cyt c- d - as with ark and dronc - regulates scutellar bristle number, which is known to depend on caspase activity. Collectively, our results indicate a role of Cyt c in caspase regulation of Drosophila somatic cells.