Fiber optic confocal reflectance microscopy:: a new real-time technique to view nuclear morphology in cervical squamous epithelium in vivo
Fiber optic confocal reflectance microscopy:: a new real-time technique to view nuclear morphology in cervical squamous epithelium in vivo
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DOI：
10.1364/oe.11.003171
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发表时间：
2003-12-01
期刊：
影响因子：
3.8
通讯作者：
Descour, MR
中科院分区：
文献类型：
--
作者：
Sung, KB;Richards-Kortum, R;Descour, MR
我们提出了一种光纤共聚焦反射显微镜(FCRM)，它可以用来对活体上皮组织进行亚细胞分辨率的成像。18例接受镜检查的患者活体获得了正常和异常宫颈组织的共焦图像；活检标本取自成像部位。该系统横向分辨率为1.6微米，轴向分辨率为3微米。形态特征，包括核大小和核浆比，从不同深度的上皮表面下获得的共聚焦图像中提取。从共聚焦图像中提取的图像特征与从体外获得的共聚焦图像和先前的组织病理学研究中提取的特征进行了很好的比较。这项研究表明，光纤共聚焦反射显微镜可以用来显示活体宫颈上皮的形态。(C)2003年美国光学学会。
We present a fiber optic confocal reflectance microscope (FCRM) which can be used to image epithelial tissue with sub-cellular resolution in vivo. Confocal images of normal and abnormal appearing cervical tissue were obtained in vivo from eighteen patients undergoing colposcopic examination of the cervix; biopsy specimens were taken from imaged sites. The measured lateral and axial resolutions of the system were 1.6 mum and 3 mum, respectively. Morphologic features, including nuclear size and nuclear-to-cytoplasmic ratio, were extracted from confocal images obtained at various depths beneath the epithelial surface. Image features extracted from confocal images compared well with features extracted from confocal images obtained in vitro and from previous histopathologic studies. This study shows that fiber optic confocal reflectance microscopy can be used to visualize the morphology of cervical epithelium in vivo. (C) 2003 Optical Society of America.