Association of the Leptin Gene with Carcass Characteristics in Nellore Cattle
Association of the Leptin Gene with Carcass Characteristics in Nellore Cattle
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DOI：
10.1080/10495398.2013.770008
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发表时间：
2013-07-01
影响因子：
3.7
通讯作者：
Grisolia, Alexeia Barufatti
中科院分区：
文献类型：
--
作者：
de Oliveira, Joyce Azambuja;da Cunha, Camila Magalhaes;Grisolia, Alexeia Barufatti
DNA技术的进步创造了生物技术工具，可用于动物选择和提高畜群生产力和质量的新策略。本研究的目的是将瘦素基因外显子2多态性的基因型与耐洛牛的生产性状联系起来。采集Nellore男性的血液，用限制性内切酶ClaI和Kpn2I进行PCR-RFLP反应。等位基因A和T被ClaI消化后的基因频率分别为0.60和0.40；基因型频率AA=0.20， AT=0.80。由Kpn2I消化的等位基因C和T的基因频率分别为0.81和0.194；基因型频率CC=0.62， CT=0.38。两种种群均不处于Hardy-Weinberg平衡（p < 0.05），未发现TT基因型。瘦素基因外显子2多态性与内洛尔牛的5个生产性状（胴体脂肪分布、红肌颜色强度、pH值、大理石纹和屠宰后脂肪厚度）显著相关。
Advances in DNA technology have created biotechnological tools that can be used in animal selection and new strategies for increasing herd productivity and quality. The objective of the present work was to associate the genotypes of leptin gene exon 2 polymorphisms with productive traits in Nellore cattle. Blood was collected from Nellore males and PCR-RFLP reactions were performed with the restriction enzymes ClaI and Kpn2I. The gene frequencies resulting from digestion by ClaI were 0.60 and 0.40 for allele A and T, respectively; the genotypic frequencies were AA=0.20 and AT=0.80. The gene frequencies from digestion by Kpn2I were 0.81 for allele C and 0.194 for allele T; the genotypic frequencies were CC=0.62 and CT=0.38. The populations in both cases were not in Hardy-Weinberg equilibrium (p>0.05), and the TT genotype was not found. Significant associations were noted between leptin gene exon 2 polymorphisms and five productive traits in Nellore cattle: carcass fat distribution, the intensity of red muscle coloration, pH, marbling, and post-slaughter fat thickness.