Flexible method for generating needle-shaped beams and its application in optical coherence tomography.

Flexible method for generating needle-shaped beams and its application in optical coherence tomography.
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DOI:
10.1364/optica.456894
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发表时间:
2022-08-20
期刊:
影响因子:
10.4
通讯作者:
--
中科院分区:
物理与天体物理1区
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--
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针状光束(NBS)具有长焦深(DOF)的特点,可以极大地提高显微系统的分辨率。然而,到目前为止,由于缺乏通用的、灵活的生成方法,特定NB的实现一直是繁重的。在这里,我们开发了一种空间多路复用位相图案,它创建了许多轴向紧密间隔的焦点,作为定制各种NBS的通用平台,允许灵活地操纵光束长度和直径、均匀的轴向强度和次衍射极限光束。用这种方法设计的NBS成功地扩展了我们光学相干层析成像(OCT)系统的自由度。它显示了整个人类表皮清晰的个别表皮细胞,大深度范围内人类真皮-表皮交界处的精细结构,以及活的果蝇幼虫的高分辨率动态心跳。
Needle-shaped beams (NBs) featuring a long depth-of-focus (DOF) can drastically improve the resolution of microscopy systems. However, thus far, the implementation of a specific NB has been onerous due to the lack of a common, flexible generation method. Here we develop a spatially multiplexed phase pattern that creates many axially closely spaced foci as a universal platform for customizing various NBs, allowing flexible manipulations of beam length and diameter, uniform axial intensity, and sub-diffraction-limit beams. NBs designed via this method successfully extended the DOF of our optical coherence tomography (OCT) system. It revealed clear individual epidermal cells of the entire human epidermis, fine structures of human dermal-epidermal junction in a large depth range, and a high-resolution dynamic heartbeat of alive Drosophila larvae.
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