Versatile, fully automated, microfluidic cell culture system
Versatile, fully automated, microfluidic cell culture system
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DOI：
10.1021/ac071311w
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发表时间：
2007-11-15
影响因子：
7.4
通讯作者：
Quake, Stephen R.
中科院分区：
文献类型：
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作者：
Gomez-Sjoeberg, Rafael;Leyrat, Anne A.;Quake, Stephen R.
在干细胞生物学的激烈活动和系统生物学的出现的推动下，对自动化和定量细胞培养技术的需求不断增加。我们建立了一个基于微流控芯片的全自动细胞培养筛选系统，该系统可以在96个独立的培养室中创建任意的培养基配方，并保持细胞活力数周。根据细胞接种密度、培养液成分和进料程序定制单独的培养条件，并用延时显微镜对每个小室进行成像。使用该装置，我们首次定量测量了瞬时刺激方案对人原代间充质干细胞的增殖、成骨分化和运动能力的影响。
There is increasing demand for automated and quantitative cell culture technology, driven both by the intense activity in stem cell biology and by the emergence of systems biology. We built a fully automated cell culture screening system based on a microfluidic chip that creates arbitrary culture media formulations in 96 independent culture chambers and maintains cell viability for weeks. Individual culture conditions are customized in terms of cell seeding density, composition of culture medium, and feeding schedule, and each chamber is imaged with time-lapse microscopy. Using this device, we perform the first quantitative measurements of the influence of transient stimulation schedules on the proliferation, osteogenic differentiation, and motility of human primary mesenchymal stem cells.