Similarity of mouse perivascular and brown adipose tissues and their resistance to diet-induced inflammation
Similarity of mouse perivascular and brown adipose tissues and their resistance to diet-induced inflammation
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DOI：
10.1152/ajpheart.00376.2011
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发表时间：
2011-10-01
影响因子：
4.8
通讯作者：
Czech, Michael P.
中科院分区：
文献类型：
--
作者：
Fitzgibbons, Timothy P.;Kogan, Sophia;Czech, Michael P.
[10]李文辉，李文辉.小鼠血管周围和棕色脂肪组织的相似性及其对饮食诱导炎症的抵抗力。Am J Physiol Heart Circ Physiol 301：H1425-H1437，2011.首次发表于2011年7月15日; doi：10.1152/ajpheart.00376.2011。胸血管周围脂肪组织（PVAT）是一种独特的脂肪储存库，可能影响血管功能和肥胖和代谢综合征发病机制的易感性。令人惊讶的是，据报道PVAT具有棕色和白色脂肪的共同特征，但尚未与肩胛间棕色脂肪组织（BAT）进行详细的直接比较。在这里，我们通过全基因组DNA微阵列分析表明，PVAT的全球基因表达谱几乎与BAT相同，Ucp-1，Cidea和其他已知在BAT中独特或非常高表达的基因的表达同样高。PVAT和BAT在免疫组化分析中也显示出几乎相同的表型，电子显微镜证实PVAT含有多室脂滴和丰富的线粒体。与白色脂肪组织（WAT）相比，来自喂食高脂饮食13周的C57 BL 6/J小鼠的PVAT和BAT具有显著较低的免疫细胞富集mRNA表达，表明对肥胖诱导的炎症具有抵抗力。事实上，BAT和PVAT对肥胖小鼠巨噬细胞标志物（F4/80和CD 68）的染色显示几乎没有巨噬细胞浸润，BAT的FACS分析证实，与WAT（31%）相比，BAT（1.0%）中存在非常少的CD 11b（+）/CD 11 c（+）巨噬细胞。总之，来自胸主动脉的小鼠PVAT与肩胛间BAT几乎相同，对饮食诱导的巨噬细胞浸润具有抗性，因此可能在保护血管床免受炎症应激方面发挥重要作用。
Fitzgibbons TP, Kogan S, Aouadi M, Hendricks GM, Straubhaar J, Czech MP. Similarity of mouse perivascular and brown adipose tissues and their resistance to diet-induced inflammation. Am J Physiol Heart Circ Physiol 301: H1425-H1437, 2011. First published July 15, 2011; doi: 10.1152/ajpheart.00376.2011.-Thoracic perivascular adipose tissue (PVAT) is a unique adipose depot that likely influences vascular function and susceptibility to pathogenesis in obesity and the metabolic syndrome. Surprisingly, PVAT has been reported to share characteristics of both brown and white adipose, but a detailed direct comparison to interscapular brown adipose tissue (BAT) has not been performed. Here we show by full genome DNA microarray analysis that global gene expression profiles of PVAT are virtually identical to BAT, with equally high expression of Ucp-1, Cidea, and other genes known to be uniquely or very highly expressed in BAT. PVAT and BAT also displayed nearly identical phenotypes upon immunohistochemical analysis, and electron microscopy confirmed that PVAT contained multilocular lipid droplets and abundant mitochondria. Compared with white adipose tissue (WAT), PVAT and BAT from C57BL6/J mice fed a high-fat diet for 13 wk had markedly lower expression of immune cell-enriched mRNAs, suggesting resistance to obesity-induced inflammation. Indeed, staining of BAT and PVAT for macrophage markers (F4/80 and CD68) in obese mice showed virtually no macrophage infiltration, and FACS analysis of BAT confirmed the presence of very few CD11b(+)/CD11c(+) macrophages in BAT (1.0%) compared with WAT (31%). In summary, murine PVAT from the thoracic aorta is virtually identical to interscapular BAT, is resistant to diet-induced macrophage infiltration, and thus may play an important role in protecting the vascular bed from inflammatory stress.