Isoform-specific cDNAs for human embryonic, neonatal, and slow skeletal myosin heavy chains.
Isoform-specific cDNAs for human embryonic, neonatal, and slow skeletal myosin heavy chains.
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人类胚胎、新生儿和慢骨骼肌球蛋白重链的异构体特异性 cDNA。
DOI：
10.1111/j.1749-6632.1990.tb42370.x
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发表时间：
1990
影响因子：
5.2
通讯作者：
Rubinstein,NA
中科院分区：
文献类型：
--
作者：
Stedman,HH;Kelly,AM;Rubinstein,NA
从人胎儿肌肉 cDNA 表达文库中分离出一系列编码人慢/β、新生儿和胚胎肌球蛋白重链 (MHC) 亚型的免疫可检测部分的 cDNA 片段。在二次筛选中分离出的 6 kb 片段代表了编码全长脊椎动物 MHC（人类胚胎亚型）的第一个克隆 cDNA。在3'-非翻译区，人类和大鼠的直向同源cDNA之间存在70-80%的核苷酸序列同源性，而旁系同源cDNA之间的同源性低于65%。此外，在胚胎转录本的 5' 末端观察到大致相同水平的非翻译序列保守性。这些结果表明，对于 3'-和 5'-非翻译域，进化序列分歧的速率受到功能限制的限制。
A series of cDNA fragments encoding immunodetectable portions of the human slow/beta, neonatal, and embryonic isoforms of myosin heavy chain (MHC) were isolated from a human fetal muscle cDNA expression library. A 6 kb fragment isolated on a secondary screen represents the first cloned cDNA encoding a full-length vertebrate MHC (the human embryonic isoform). In the 3'-untranslated regions, 70-80% nucleotide sequence homology exists among orthologous human and rat cDNAs, whereas the homology is less than 65% among the paralogous cDNAs. Furthermore, approximately the same level of untranslated sequence conservation is observed at the 5'-terminus of the embryonic transcript. These results suggest that for both the 3'-and the 5'-untranslated domains, the rate of evolutionary sequence divergence is limited by functional constraints.