Idiotypic markers of polyclonal B cell activation. Public idiotypes shared by monoclonal antibodies derived from patients with systemic lupus erythematosus or leprosy.
Idiotypic markers of polyclonal B cell activation. Public idiotypes shared by monoclonal antibodies derived from patients with systemic lupus erythematosus or leprosy.
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多克隆 B 细胞激活的独特型标记。
DOI：
10.1172/jci112849
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Schwartz,RS
中科院分区：
文献类型：
--
作者：
Mackworth-Young,C;Sabbaga,J;Schwartz,RS
我们研究了来自多克隆B细胞活化患者的单克隆抗体的独特型标志物。本文报道了4株来源于瘤型麻风的单克隆抗体和3株来源于SLE患者的抗DNA单克隆抗体。三个新的公共独特位，这是共同的单克隆抗体从所有三个病人，被定义为五个多克隆兔抗独特型，两个单克隆小鼠抗独特位，和单克隆小鼠抗体的合成肽，含有残基的重链CDR-1的单克隆狼疮抗DNA抗体。发现针对合成独特型的抗体与溶液中的天然免疫球蛋白反应。发现一个独特位在所有试验动物中具有一致的免疫原性。由于这三个病人是不同的种族起源，这些共同的独特型可能是由生殖系V基因编码。这些基因可能在多克隆B细胞活化状态下反复表达，而与病因无关。结果表明，一些自身抗体的出现，在正常的抗体库的前体池的扩展。
We investigated idiotypic markers of monoclonal antibodies derived from patients with polyclonal B-cell activation. Four monoclonal antibodies with different ligand binding specificities derived from a patient with lepromatous leprosy and three monoclonal anti-DNA antibodies from two patients with SLE were studied. Three new public idiotopes, which were common to monoclonal antibodies from all three patients, were defined by five polyclonal rabbit antiidiotypes, two monoclonal mouse antiidiotopes, and a monoclonal mouse antibody against a synthetic peptide that contains residues of the heavy chain CDR-1 of a monoclonal lupus anti-DNA antibody. The antibody against the synthetic idiotype was found to react with native immunoglobulins in solution. One idiotope was found to be consistently immunogenic in all animals tested. Since the three patients are of different ethnic origins, these shared idiotypes are probably encoded by germline V genes. These genes may be recurrently expressed in states of polyclonal B-cell activation, regardless of etiology. The results suggest that some autoantibodies arise by expansion of a pool of precursors in the normal antibody repertoire.