A multifunctional ATP-binding cassette transporter system from Vibrio cholerae transports vibriobactin and enterobactin
A multifunctional ATP-binding cassette transporter system from Vibrio cholerae transports vibriobactin and enterobactin
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DOI：
10.1128/jb.181.24.7588-7596.1999
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发表时间：
1999-12-01
影响因子：
3.2
通讯作者：
Payne, SM
中科院分区：
文献类型：
--
作者：
Wyckoff, EE;Valle, AM;Payne, SM
霍乱弧菌使用邻苯二酚铁载体弧菌素在铁限制条件下运输铁。我们已经确定了弧菌毒素运输的基因，并将其定位在弧菌毒素生物合成基因簇内。在该遗传区域内，我们已经确定了四个基因，viuP，viuD，viuG和viuC，其蛋白产物分别与其他铁转运系统的周质结合蛋白，两个完整的细胞质膜蛋白和ATP酶组分具有同源性。ViuP的氨基末端区域与脂蛋白信号序列具有同源性，并且ViuP可以用[H-3]棕榈酸标记。这表明ViuP是膜脂蛋白。ViuPDGC系统在大肠杆菌中转运弧菌菌素和肠杆菌素。在同一试验中，E.虽然整个viuPDGC系统可以互补fepB、fepD、fepG或fepC中的突变，但只有viuC能够独立地互补相应的fep突变。这表明这些蛋白质通常作为复合物发挥作用。通过标记交换构建携带viuP或viuG突变的霍乱弧菌菌株。这些突变减少了，但没有完全消除，弧菌素的利用。这表明除了viuPDGC之外，霍乱弧菌还含有在儿茶酚铁载体的转运中起作用的基因。
Vibrio cholerae uses the catechol siderophore vibriobactin for iron transport under iron-limiting conditions. We have identified genes for vibriobactin transport and mapped them within the vibriobactin biosynthetic gene cluster. Within this genetic region we have identified four genes, viuP, viuD, viuG and viuC, whose protein products have homology to the periplasmic binding protein, the two integral cytoplasmic membrane proteins, and the ATPase component, respectively, of other iron transport systems, The amino-terminal region of ViuP has homology to a lipoprotein signal sequence, and ViuP could be labeled with [H-3] palmitic acid. This suggests that ViuP is a membrane lipoprotein. The ViuPDGC system transports both vibriobactin and enterobactin in Escherichia coli. In the same assay, the E. coli enterobactin transport system, FepBDGC, allowed the utilization of enterobactin but not vibriobactin, Although the entire viuPDGC system could complement mutations in fepB, fepD,fepG, or fepC only viuC was able to independently complement the corresponding fep mutation. This indicates that these proteins usually function as a complex. V. cholerae strains carrying a mutation in viuP or in,in viuG were constructed by marker exchange. These mutations reduced, but did not completely eliminate, vibriobactin utilization. This suggests that V. cholerae contains genes in addition to viuPDGC that function in the transport of catechol siderophores.