Graphene oxide arrays for detecting specific DNA hybridization by fluorescence resonance energy transfer
Graphene oxide arrays for detecting specific DNA hybridization by fluorescence resonance energy transfer
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DOI：
10.1016/j.bios.2010.02.022
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发表时间：
2010-06-15
影响因子：
12.6
通讯作者：
Seo, Tae Seok
中科院分区：
文献类型：
--
作者：
Liu, Fei;Choi, Jong Young;Seo, Tae Seok
氧化石墨烯（GO）的独特性质，如亲水性，多功能表面改性和光致发光，使它们适合于生物应用。在这项研究中，我们探索了使用GO片作为一种新型的DNA生物传感器，通过应用GO阵列格式识别特定的DNA-DNA杂交相互作用。当通过使用碳二亚胺化学连接到GO表面的探针DNA与金纳米颗粒（Au NP）标记的互补DNA链杂交时，GO阵列的荧光发射强度急剧降低。TEM数据显示，Au NPs分散在GO表面上，特别是在边缘和杂交后的折叠结构处，其密度类似于每μ m 80个Au NPs（2）。这导致CA。作为Au NP和GO片之间的荧光能量转移的结果，87%的荧光猝灭。这些结果表明，GO纳米材料，这是容易从廉价的石墨源大规模合成，可以在生物传感器，分子成像和纳米生物技术领域的广泛的生物应用。(C)2010 Elsevier B. V.保留所有权利。
The unique properties of graphene oxides (GO) such as water dispersibility, versatile surface modification, and photoluminescence make them suitable for biological applications. In this study, we explored the use of GO sheets as a novel DNA biosensor by applying the GO in an array format to recognize specific DNA-DNA hybridization interaction. When the probe DNA linked to the surface of GO by using carbodiimide chemistry is hybridized with a gold nanoparticle (Au NP) labeled complementary DNA strand, the fluorescence emission intensity of the GO array is drastically reduced. TEM data reveal that the Au NPs are dispersed on the GO surface, particularly at edges and folded structures upon hybridization with a density of similar to 80 Au NPs per mu m(2). This leads to ca. 87% fluorescence quenching as a consequence of fluorescence energy transfer between Au NPs and the GO sheets. These results suggest that the GO nanomaterials, which are readily synthesized on a large scale from a cheap graphite source, could have a wide range of bioapplications in the fields of biosensors, molecular imaging and nanobiotechnology. (C) 2010 Elsevier B.V. All rights reserved.