Diacylglycerol causes Ca release from the platelet dense tubular system: comparisons with Ca release caused by inositol 1,4,5-triphosphate.
Diacylglycerol causes Ca release from the platelet dense tubular system: comparisons with Ca release caused by inositol 1,4,5-triphosphate.
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二酰基甘油导致血小板致密管状系统释放 Ca：与肌醇 1,4,5-三磷酸引起的 Ca 释放进行比较。
DOI：
10.1016/0006-291x(87)90444-x
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发表时间：
1987
影响因子：
3.1
通讯作者：
Laposata,M
中科院分区：
文献类型：
--
作者：
Brass,LF;Laposata,M
血小板活化通常与胞质游离Ca浓度的增加有关，这部分是由于致密小管系统的Ca释放。目前的研究检查是否形成的磷脂酰肌醇水解血小板活化过程中的甘油二酯有助于这一进程。甘油二酯对致密小管系统的影响是使用用皂苷透化的血小板来测试的，然后允许其积累45 Ca。合成的二酰基甘油，1-油酰基-2-乙酰基甘油（OAG），释放高达70%的离子载体A23187-可释放的45 Ca，在相同的条件下，释放的比例相同的肌醇1，4，5-三磷酸（IP 3）。45 Ca的释放是在40 μM OAG和1 μM IP 3的一半最大。对OAG的反应不受阿司匹林的抑制，并且不能通过添加佛波酯来再现，这表明它既不涉及花生四烯酸代谢，也不涉及蛋白激酶C激活。OAG诱导的45 Ca释放的时间过程，这是慢于IP 3诱导的45 Ca释放，对应于OAG转化为1-油酰基-2-乙酰酰基磷脂酸（OAG-PA）的时间过程。当OAG-PA或溶血磷脂酸加入到皂苷处理的血小板中时，45 Ca释放的程度与OAG相似，但OAG-PA和溶血磷脂酸的效力比OAG高5至10倍（以摩尔计）。这些数据表明：（1）甘油二酯引起的钙释放实际上是由于磷脂酸和/或溶血磷脂酸的形成，（2）这些分子不是作为简单的钙离子载体，（3）甘油二酯代谢物可能增加血小板活化过程中IP 3引起的钙稳态变化。
Platelet activation is often associated with an increase in the cytosolic free Ca concentration that is due in part to Ca release from the dense tubular system. The present studies examine whether the diacylglycerol formed by phosphoinositide hydrolysis during platelet activation contributes to this process. The effect of diacylglycerol on the dense tubular system was tested using platelets that were permeabilized with saponin and then allowed to accumulate45Ca. A synthetic diacylglycerol, 1-oleoyl-2-acetoyl glycerol (OAG), released up to 70% of the ionophore A23187-releasable45Ca, a fraction identical to that discharged by inositol 1,4,5-triphosphate (IP3) under the same conditions.45Ca release was half-maximal at 40 μM OAG and 1 μM IP3. The response to OAG was not inhibited by aspirin and could not be reproduced by the addition of a phorbol ester, which suggests that it involves neither arachidonic acid metabolism nor protein kinase C activation. The time course of OAG-induced45Ca release, which was slower than IP3-induced45Ca release, corresponded to the time course of conversion of the OAG to 1-oleoyl-2-acetoyl phosphatidic acid (OAG-PA). When either OAG-PA or lysophosphatidic acid was added to the saponin-treated platelets, the extent of45Ca release was similar to that observed with OAG, but both the OAG-PA and the lysophosphatidic acid were 5 to 10 times more potent than OAG on a molar basis. These data suggest: (1) that the Ca release caused by diacylglycerol is actually due to formation of phosphatidic acid and/or lysophosphatidic acid, (2) that these molecules are not acting as simple Ca ionophores and (3) that diacylglycerol metabolites may augment the changes in Ca homeostasis caused by IP3during platelet activation.
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sn-1,2-二酰基甘油第二信使的减弱。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bishop,WR;Bell,RM
通讯作者：
Bell,RM
溶血磷脂酸的血小板聚集活性与其钙离子载体特性无关
影响因子：
3.5
作者：
M. Simon;H. Chap;L. Douste‐Blazy
通讯作者：
L. Douste‐Blazy
肌醇 1,4,5-三磷酸诱导高度纯化的人血小板细胞内膜中隔离的 Ca2 的释放。
影响因子：
4.1
作者：
K. Authi;N. Crawford
通讯作者：
N. Crawford
凝血酶刺激的血小板中磷脂酰肌醇 4,5-二磷酸快速减少。
影响因子：
4.8
作者：
M. Billah;E. Lapetina
通讯作者：
E. Lapetina
使用二酰基甘油激酶定量 1,2-二酰基甘油的皮摩尔水平。
影响因子：
2.9
作者：
D. Kennerly;C. Parker;T. Sullivan
通讯作者：
T. Sullivan