Structural analysis of photosynthetic membranes by cryo-electron tomography of intact Rhodopseudomonas viridis cells
Structural analysis of photosynthetic membranes by cryo-electron tomography of intact Rhodopseudomonas viridis cells
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DOI：
10.1016/j.jsb.2007.09.014
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发表时间：
2008-03-01
影响因子：
3
通讯作者：
Medalia, Ohad
中科院分区：
文献类型：
--
作者：
Konorty, Marina;Kahana, Nava;Medalia, Ohad
在光合作用过程中，高度组织化的膜组件将光高效地转化为生化能。我们已经使用了整装冷冻电子断层扫描研究的细胞内结构的光合膜的厌氧紫色光合细菌Rhodopyreneaviridis，以及，作为组织内的膜的光合单位。三维重建显示质膜与光合膜的连续性，光合膜在连接位点形成平均直径为31 nm +/- 8 nm的隧道状结构。光合膜在其细胞质面之间的间距被发现是11纳米，从而执行一个高度紧密的包装的光合膜。连续断层切片分析允许推导相邻的光合作用的核心复合物之间的间距从单层光合作用膜，原位。这一分析表明，大多数，如果不是全部，光合膜在R。绿色的特征在于类似的二维六边形晶格组织。(C)2007爱思唯尔公司All rights reserved.
During the photosynthetic process, highly organized membranal assemblies convert light into biochemical energy with high efficiency. We have used whole-mount cryo-electron tomography to study the intracellular architecture of the photosynthetic membranes of the anaerobic purple photosynthetic bacterium Rhodopseudomonas viridis, as well,as the organization of the photosynthetic units within the membranes. Three-dimensional reconstruction demonstrates a continuity of the plasma membrane with the photosynthetic membranes that form tunnel-like structures with an average diameter of 31 nm +/- 8 nm at the connection sites. The spacing between the photosynthetic membranes at their cytoplasmic faces was found to be 11 nm, thus enforcing a highly close packaging of the photosynthetic membranes. Analysis of successive tomographic slices allowed for derivation of the spacing between adjacent photosynthetic core complexes from a single-layered photosynthetic membrane, in situ. This analysis suggests that most, if not all, photosynthetic membranes in R. viridis are characterized by a similar two-dimensional hexagonal lattice organization. (C) 2007 Elsevier Inc. All rights reserved.