Stability and kinetics of G-quadruplex structures.
Stability and kinetics of G-quadruplex structures.
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DOI：
10.1093/nar/gkn517
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发表时间：
2008-10
影响因子：
14.9
通讯作者：
Trent JO
中科院分区：
文献类型：
--
作者：
Lane AN;Chaires JB;Gray RD;Trent JO
在这篇综述中，我们给出了一个概述的结构和稳定性的单分子富含G的四链体结构，相关的药物设计和体内功能的最新文献。在这次审查的统一主题是能量。四链体的热力学稳定性还没有像DNA和RNA双链体那样详细地研究过，并且这些类别的折叠寡核苷酸之间的力平衡存在重要差异。我们提供了稳定性原则的概述，并在可用的实验数据，这些原则的报告。文献中的重大空白已被确定，应通过明确定义的四链体的系统性研究来填补，不仅为设计目的提供稳定性的基本理解，而且因为它涉及四链体的体内发生。通常适用于确定的结构，稳定性和折叠的技术进行了讨论的信息内容和限制。四链体结构折叠和展开相对较慢，与转录和复制相关的DNA解旋事件可能远离平衡运行。四链体的形成和分辨率的动力学，和方法论的稳定性和它们可能的生物发生的背景下进行了讨论。
In this review, we give an overview of recent literature on the structure and stability of unimolecular G-rich quadruplex structures that are relevant to drug design and for in vivo function. The unifying theme in this review is energetics. The thermodynamic stability of quadruplexes has not been studied in the same detail as DNA and RNA duplexes, and there are important differences in the balance of forces between these classes of folded oligonucleotides. We provide an overview of the principles of stability and where available the experimental data that report on these principles. Significant gaps in the literature have been identified, that should be filled by a systematic study of well-defined quadruplexes not only to provide the basic understanding of stability both for design purposes, but also as it relates to in vivo occurrence of quadruplexes. Techniques that are commonly applied to the determination of the structure, stability and folding are discussed in terms of information content and limitations. Quadruplex structures fold and unfold comparatively slowly, and DNA unwinding events associated with transcription and replication may be operating far from equilibrium. The kinetics of formation and resolution of quadruplexes, and methodologies are discussed in the context of stability and their possible biological occurrence.
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四分子G四链体的形成途径。
影响因子：
14.9
作者：
Bardin, Caroline;Leroy, Jean Louis
通讯作者：
Leroy, Jean Louis
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TINOCO, I
影响因子：
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