FINE-STRUCTURE MAPPING OF AN AVIAN TUMOR-VIRUS RNA BY IMMUNOELECTRON MICROSCOPY
FINE-STRUCTURE MAPPING OF AN AVIAN TUMOR-VIRUS RNA BY IMMUNOELECTRON MICROSCOPY
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DOI：
10.1093/nar/8.19.4485
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发表时间：
1980-01-01
影响因子：
14.9
通讯作者：
ERLANGER, BF
中科院分区：
文献类型：
--
作者：
CASTLEMAN, H;MEREDITH, RD;ERLANGER, BF
通过新开发的使用抗核苷酸抗体作为探针的免疫电子显微镜方法检查了禽肉瘤病毒（ASV）缺失株（缺乏Src基因）的RNA。在RNA变性并与高亲和力、高特异性的抗7-甲基鸟苷-5 ′-磷酸（抗pm 7 G）反应后，发现在检测的106个分子中，81%的分子在一端具有抗体，这与ASV-RNA中存在pm 7 G帽一致。可以证明pm 7 G对半抗原的抑制。抗A和抗poly A的实验结果与ASV-RNA的已知结构一致，特别是3′ poly A尾的存在。这些研究说明了在核酸精细结构的联合免疫化学和电子显微镜研究中使用抗核苷酸抗体的可行性。
The RNA of a deleted strain (lacking Src gene) of an avian sarcoma virus (ASV) was examined by a newly developed immunoelectron microscopic procedure which uses anti-nucleotide antibodies as probes. After denaturation of the RNA and reaction with a high affinity, highly specific anti-7-methylguanosine-5′-phosphate (anti-pm7G), 81% of 106 molecules examined were found to have antibody at one terminus, in agreement with the presence of a pm7G cap in ASV-RNA. Hapten inhibition by pm7G could be demonstrated. Experiments with anti-A and with anti-poly A gave results consistent with the known structure of ASV-RNA, in particular the presence of a 3′ poly A tail. These studies illustrate the feasibility of using anti-nucleotide antibodies in a combined immunochemical and electron microscopic study of the fine structure of nucleic acids.