Comparison of Two Experimental Models for Urethral Stricture in the Anterior Urethra of the Male Rabbit
Comparison of Two Experimental Models for Urethral Stricture in the Anterior Urethra of the Male Rabbit
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DOI：
10.1016/j.urology.2012.04.025
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发表时间：
2012-07-01
期刊：
影响因子：
2.1
通讯作者：
Ozgul, Aydin
中科院分区：
文献类型：
--
作者：
Faydaci, Gokhan;Tarhan, Fatih;Ozgul, Aydin
目的比较两种尿道狭窄模型的优缺点，确定建立兔尿道狭窄模型的适宜方法。随机分为3组。使用小儿电切镜（13 F）进行尿道镜检查。在1组（10只家兔）中，对前尿道进行2-3 mm长的环形电凝，直至发生粘膜溃疡。在第二组（10只兔子）中，从前尿道进行2-3 mm宽的切除，切除的深度足以暴露尿道周围组织，以允许尿液从管腔泄漏。另8只家兔作为对照组，仅行尿道镜检查。第30天行逆行尿道造影和尿道镜检查，以评估尿道狭窄的形成。结果电凝组和对照组均未出现尿道狭窄，电切组尿道狭窄明显，电凝组尿道狭窄明显，电切组尿道狭窄明显，电凝组尿道狭窄明显，电切组尿道狭窄明显。电凝组6只兔尿道腔侧见再生尿道，4只兔尿道正常。电切组尿道腔内广泛纤维化阻塞，尿道壁胶原纤维束结节状增生。结论电切法较电凝法能更有效地治疗兔尿道狭窄。从前尿道切除2-3毫米宽的部分，可以成功地产生尿道狭窄的电凝。电切术是一种理想的尿道狭窄动物模型。泌尿学80：225.e7-225.e11，2012年。(C)2012 Elsevier Inc.
OBJECTIVE To compare 2 urethral stricture models and determine the appropriate model for the generation of urethral stricture in rabbits.METHODS A total of 28 adult male New Zealand rabbits were included in the present study. They were separated randomly into 3 groups. Urethroscopy was performed using a pediatric resectoscope (13F). In 1 group (10 rabbits), circumferential electrocoagulation of the anterior urethra 2-3 mm long was performed until ulceration of the mucosa occurred. In the second group (10 rabbits), 2-3 mm wide resection from the anterior urethra was performed that was deep enough to expose the periurethral tissue to allow urine leakage from the lumen. The remaining 8 rabbits as the control group underwent only urethroscopy. On the 30th day, retrograde urethrography and urethroscopy were performed to evaluate urethral stricture formation. Histologic examination was done to evaluate the urethral pathologic changes.RESULTS According to the urethrography and urethroscopy findings, no rabbits in the electrocoagulation or control group developed urethral stricture; however, significant stricture formation was observed in every case of the electroresection group. Regenerating urothelium at the luminal side of the urethra was seen in 6 rabbits in the electrocoagulation group and 4 showed normal urethra. In the electroresection group, extensive fibrosis that obstructed the urethral lumen and nodular collagen bundles in the urethral wall were seen.CONCLUSION The electroresection method is more reliable than the electrocoagulation method in developing urethral stricture in the rabbit model. A 2-3-mm-wide resection from the anterior urethra can successfully generate urethral stricture with respect to electrocoagulation. Electroresection offers an ideal animal model for generating urethral stricture. UROLOGY 80: 225.e7-225.e11, 2012. (C) 2012 Elsevier Inc.