The Aquilegia JAGGED homolog promotes proliferation of adaxial cell types in both leaves and stems.
The Aquilegia JAGGED homolog promotes proliferation of adaxial cell types in both leaves and stems.
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DOI：
10.1111/nph.14282
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发表时间：
2017-10
期刊：
影响因子：
--
通讯作者：
Ya Min;E. Kramer
中科院分区：
文献类型：
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作者：
Ya Min;E. Kramer
为了探讨JAGGED基因的功能保守性，我们在低等真双子叶植物天蓝Aquilegia coerulea中鉴定了其直系同源基因AqJAG。我们分析了AqJAG在不同组织和发育阶段的表达模式，并使用基于RNAi的方法来产生AqJAG的敲低表型。AqJAG在茎尖、花分生组织、侧根原基和所有侧生器官原基中均有强表达。AqJAG的沉默揭示了发育中的茎、叶和花的广泛缺陷;最强的表型包括由于细胞大小和数量减少而导致的小叶片层严重减少、近轴细胞身份的改变、花序茎上片状组织的生长，以及花分生组织和花器官原基的早期停滞。我们的研究结果表明，AqJAG在控制花器官的原基起始和远端生长以及小叶的层状发育中起着关键作用。最引人注目的是，我们证明了AqJAG主动控制营养组织中具有近轴身份的细胞的行为，为细胞增殖如何以身份特异性方式控制提供了证据。
In order to explore the functional conservation of JAGGED, a key gene involved in the sculpting of lateral organs in several model species, we identified its ortholog AqJAG in the lower eudicot species Aquilegia coerulea. We analyzed the expression patterns of AqJAG in various tissues and developmental stages, and used RNAi-based methods to generate knockdown phenotypes of AqJAG. AqJAG was strongly expressed in shoot apices, floral meristems, lateral root primordia and all lateral organ primordia. Silencing of AqJAG revealed a wide range of defects in the developing stems, leaves and flowers; strongest phenotypes include severe reduction of leaflet laminae due to a decrease in cell size and number, change of adaxial cell identity, outgrowth of laminar-like tissue on the inflorescence stem, and early arrest of floral meristems and floral organ primordia. Our results indicate that AqJAG plays a critical role in controlling primordia initiation and distal growth of floral organs, and laminar development of leaflets. Most strikingly, we demonstrated that AqJAG disproportionally controls the behavior of cells with adaxial identity in vegetative tissues, providing evidence of how cell proliferation is controlled in an identity-specific manner.