Brown-midrib maize (bm1) -: a mutation affecting the cinnamyl alcohol dehydrogenase gene
Brown-midrib maize (bm1) -: a mutation affecting the cinnamyl alcohol dehydrogenase gene
复制标题
DOI：
10.1046/j.1365-313x.1998.00153.x
复制
发表时间：
1998-06-01
期刊：
影响因子：
7.2
通讯作者：
Foxon, GA
中科院分区：
文献类型：
--
作者：
Halpin, C;Holt, K;Foxon, GA
玉米的棕色中脉突变体具有红棕色的改性木质素。虽然四个独立的bm基因座是已知的，只有一个突变基因已被确定。我们在这里报告说，玉米BM1，较少表征的突变体之一，显示出严重降低CAD活性在木质素组织，导致生产的改性木质素。总木质素含量和聚合物的结构都通过突变而改变。我们进一步描述了玉米CAD cDNA的分离和表征以及CAD基因的定位。CAD地图非常密切的已知位置的bm1和共分离的bm1位点在两个独立的重组近交群体。这些数据有力地支持了玉米bm1直接影响CAD基因表达的前提。
Brown-midrib (bm) mutants of maize have modified lignin of reddish-brown colour. Although four independent bm loci are known, only one of the mutant genes has been previously identified. We report here that maize bm1, one of the less characterised mutants, shows severely reduced CAD activity in lignified tissues, resulting in the production of a modified lignin. Both the total lignin content and the structure of the polymer are altered by the mutation. We further describe the isolation and characterisation of the maize CAD cDNA and mapping of the CAD gene. CAD maps very closely to the known location of bm1 and co-segregates with the bm1 locus in two independent recombinant inbred populations. These data strongly support the premise that maize bm1 directly affects expression of the CAD gene.