THE REGIONAL DISTRIBUTION AND CHROMATOGRAPHIC CHARACTERIZATION OF NEUROTENSIN-LIKE IMMUNOREACTIVITY IN THE RAT CENTRAL NERVOUS-SYSTEM
THE REGIONAL DISTRIBUTION AND CHROMATOGRAPHIC CHARACTERIZATION OF NEUROTENSIN-LIKE IMMUNOREACTIVITY IN THE RAT CENTRAL NERVOUS-SYSTEM
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DOI：
10.1111/j.1471-4159.1982.tb05340.x
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发表时间：
1982-01-01
影响因子：
4.7
通讯作者：
STPIERRE, S
中科院分区：
文献类型：
--
作者：
EMSON, PC;GOEDERT, M;STPIERRE, S
羧基和氨基末端特异性神经降压素抗血清的特点，并用于确定在大鼠中枢神经系统神经降压素样免疫反应的性质。使用这些抗血清，连同反相HPLC [高压液相色谱]柱上神经降压素样免疫反应性的分离，很明显，大部分大鼠CNS神经降压素样免疫反应性与合成的十三肽是不可区分的。检测到少量的羧基和氨基末端神经降压素样免疫反应性，这可能对应于神经降压素的羧基和氨基末端片段。使用氨基末端定向神经紧张素抗血清，神经紧张素样免疫反应在大鼠中枢神经系统的详细分布。含量最高的是下丘脑、中央杏仁核、终纹床核、脊髓胶状质和三叉神经区域。
Carboxy- and amino-terminal specific neurotensin antisera were characterized and used to determine the nature of neurotensin-like immunoreactivity in the rat CNS. Using these antisera, together with the separation of neurotensin-like immunoreactivity on reverse-phase HPLC [high-pressure liquid chromatography] columns, it is clear that the majority of rat CNS neurotensin-like immunoreactivity is indistinguishable from the sysnthetic tridecapeptide. Smaller amounts of carboxy- and amino-terminal neurotensin-like immunoreactivity were detected, which may correspond to carboxy- and amino-terminal fragments of neurotensin. Using the amino-terminal directed neurotension antiserum, a detailed distribution of neurotensin-like immunoreactivity in the rat CNS is described. Highest amounts were found in the hypothalamus, central amygdaloid nucleus, bed nucleus of the stria terminalis and the substantia gelatinosa of the spinal cord and of the trigeminal region.