Microarray analysis of sexually dimorphic gene expression in human minor salivary glands.
Microarray analysis of sexually dimorphic gene expression in human minor salivary glands.
复制标题
人类小唾液腺中性二态性基因表达的微阵列分析。
DOI：
10.1111/j.1601-0825.2011.01816.x
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发表时间：
2011
期刊：
影响因子：
3.8
通讯作者：
Chiorini,Ja
中科院分区：
文献类型：
--
作者：
Michael,D;Soi,S;Cabera-Perez,J;Weller,M;Alexander,S;Alevizos,I;Illei,Gg;Chiorini,Ja
口腔疾病 (2011)17, 653–661 目的：我们假设性别之间的 mRNA 转录差异可能与女性与男性发生干燥综合征 (SS) 的 9:1 性别相关相对风险有关，这是一种导致泪腺和唾液腺炎症和功能障碍的自身免疫性疾病。 对象和方法：从 9 名健康人的小唾液腺中收集 RNA志愿者（四名男性和五名女性）并使用安捷伦 4 × 44K 人类微阵列平台进行分析。通过 qRT-PCR 证实了差异表达。结果：将正常男性和女性志愿者的小唾液腺转录组与唾液腺和分泌上皮的转录组进行比较，确定了许多性别、物种和组织特异性基因表达模式。这些差异包括但不限于涉及免疫调节、趋化控制、补体抑制、代谢和神经发生的多种基因。结论：对这些变化的分析可以深入了解可能参与干燥综合征发生的保护性和诱发性分子因素。本研究中观察到的一些基因变化与之前观察到的唾液腺功能的性别二态性相关，并且还说明了需要进一步研究的几个新目标。
Oral Diseases(2011)17, 653–661Objective:We hypothesized that differential mRNA transcription between the sexes may be linked to the 9:1 female‐to‐male gender‐related relative risk for the development of Sjögren’s syndrome (SS), an autoimmune disease that leads to inflammation and dysfunction in the lachrymal and salivary glands.Subjects and Methods:RNA from minor salivary glands was collected from nine healthy volunteers (four men and five women) and analyzed using the Agilent 4 × 44K human microarray platform. Differential expression was confirmed by qRT‐PCR.Results:Comparison of the transcriptome of minor salivary glands from normal male and female volunteers with that of salivary glands and secretory epithelia identified a number of gender, species, and tissue‐specific gene expression patterns. These differences include, but are not limited to, a diverse set of genes involved in immune modulation, chemotactic control, inhibition of complement, metabolism, and neurogenesis.Conclusion:Analysis of these changes provides insight into the protective and predisposing molecular factors that may be involved in the development of Sjögren’s syndrome. Some of the gene changes observed in this study correlate with previously observed sexual dimorphisms in salivary gland function and also illustrate several new targets for further investigation.