Metabolism of antioxidant and chemopreventive ellagitannins from strawberries, raspberries, walnuts, and oak-aged wine in humans:: Identification of biomarkers and individual variability
Metabolism of antioxidant and chemopreventive ellagitannins from strawberries, raspberries, walnuts, and oak-aged wine in humans:: Identification of biomarkers and individual variability
复制标题
DOI：
10.1021/jf049144d
复制
发表时间：
2005-01-26
影响因子：
6.1
通讯作者：
Espín, JC
中科院分区：
文献类型：
--
作者：
Cerdá, B;Tomás-Barberán, FA;Espín, JC
鞣花单宁 (ET) 是膳食多酚，含有鞣花酸 (EA) 亚基，具有抗氧化和癌症化学预防活性，可能有助于人类健康。然而，人们对它们的代谢命运知之甚少。我们在此研究不同膳食 ET 和 EA 衍生物在人体中的代谢。四十名健康志愿者分为四组。每组单剂量食用不同的含 ET 食品，即草莓（250 克）、红树莓（225 克）、核桃（35 克）和橡木陈酿的红酒（300 毫升）。摄入后，在 8 (F1)、16 (F2)、32 (F3)、40 (F4) 和 56 (F5) h 收集 5 个尿液组分 (F)。 LC-MS/MS 分析后，尿液中未检测到 ET 和 EA。然而，在所有受试者的 F3-F5 级分中均检测到与葡萄糖醛酸结合的微生物代谢物 3,8-二羟基-6H-二苯并[b,d]吡喃-6-酮（尿石素 B），与食用的食品无关。就摄入的 ET 而言，代谢物排泄的平均百分比范围为 2.8%（草莓）至 16.6%（核桃）。注意到显着的个体差异，确定了每组中的“高代谢物排泄物和低代谢物排泄物”，这支持了结肠微生物群的参与。在ET代谢中。这些结果表明，尿石素 B（一种先前描述的抗血管生成和透明质酸酶抑制剂化合物）是人类暴露于膳食 ET 的生物标志物，可能有助于含 ET 产品的干预研究。应考虑膳食 ET 和 EA 在胃肠道中的抗氧化和抗癌作用，而潜在全身活性的研究应重点关注生物可利用的尿石素 B 衍生物。
Ellagitannins (ETs) are dietary polyphenols, containing ellagic acid (EA) subunits, with antioxidant and cancer chemopreventive activities that might contribute to health benefits in humans. However, little is known about their metabolic fate. We investigate here the metabolism of different dietary ETs and EA derivatives in humans. Forty healthy volunteers were distributed in four groups. Each group consumed, in a single dose, a different ET-containing foodstuff, i.e., strawberries (250 g), red raspberries (225 g), walnuts (35 g), and oak-aged red wine (300 mL). After the intake, five urine fractions (F) were collected at 8 (F1), 16 (F2), 32 (F3), 40 (F4), and 56 (F5) h. Neither ETs nor EA were detected in urine after LC-MS/MS analysis. However, the microbial metabolite 3,8-dihydroxy-6H-dibenzo[b,d]pyran-6-one (urolithin B) conjugated with glucuronic acid was detected along the fractions F3-F5 in all of the subjects, independently of the consumed foodstuff. The mean percentage of metabolite excretion ranged from 2.8 (strawberries) to 16.6% (walnuts) regarding the ingested ETs. Considerable interindividual differences were noted, identifying "high and low metabolite excreters" in each group, which supported the involvement of the colonic microflora. in ET metabolism. These results indicate that urolithin B (a previously described antiangiogenic and hyaluronidase inhibitor compound) is a biomarker of human exposure to dietary ETs and may be useful in intervention studies with ET-containing products. The antioxidant and anticarcinogenic effects of dietary ETs and EA should be considered in the gastrointestinal tract whereas the study of potential systemic activities should be focused on the bioavailable urolithin B derivatives.