Imaging cells and tissues with refractive index radiology
Imaging cells and tissues with refractive index radiology
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DOI：
10.1529/biophysj.103.034991
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发表时间：
2004-12-01
影响因子：
3.4
通讯作者：
Margaritondo, G
中科院分区：
文献类型：
--
作者：
Hwu, Y;Tsai, WL;Margaritondo, G
单个细胞，包括活细胞，可以用硬x射线成像吗？到目前为止，普遍的看法是需要复杂的染色技术来完成这项任务。相反，我们表明，单个细胞和细胞的细节可以在培养液和组织中检测到，没有染色，也没有其他对比度增强的准备。所检查的样品可以比许多其他显微镜技术厚得多，而不会牺牲分辨细胞的能力。在我们的方法中的关键因素是使用一个相干的同步辐射源和基于折射率的对比度机制。第一次成功的测试是在各种细胞系统上进行的，包括皮肤和内部叶细胞，小鼠神经元，兔成纤维细胞和人类肿瘤细胞。
Can individual cells, including live cells, be imaged using hard x rays? Common wisdom until now required sophisticated staining techniques for this task. We show instead that individual cells and cell details can be detected in culture solution and tissues with no staining and no other contrast-enhancing preparation. The sample examined can be much thicker than for many other microscopy techniques without sacrificing the capability to resolve cells. The key factor in our approach is the use of a coherent synchrotron source and of contrast mechanisms based on the refractive index. The first successful tests were conducted on a variety of cell systems including skin and internal leaf cells, mouse neurons, rabbit fibroblast cells, and human tumor cells.