A Kinase-START Gene Confers Temperature-Dependent Resistance to Wheat Stripe Rust
A Kinase-START Gene Confers Temperature-Dependent Resistance to Wheat Stripe Rust
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DOI：
10.1126/science.1166289
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发表时间：
2009-03-06
期刊：
影响因子：
56.9
通讯作者：
Dubcovsky, Jorge
中科院分区：
文献类型：
--
作者：
Fu, Daolin;Uauy, Cristobal;Dubcovsky, Jorge
条锈病是一种毁灭性的真菌疾病，在世界许多地区折磨小麦。小麦条锈菌的新小种克服了大多数已知小种特异性抗性基因。我们报告了基因Yr 36（WKS 1）的图位克隆，该基因在相对高温（25度至35度C）下对广谱条锈病小种具有抗性。该基因包括激酶和推定的START脂质结合结构域。五个独立的突变和转基因互补证实了这两个结构域是必要的赋予抗性。Yr 36存在于野生小麦中，但在现代面食和面包小麦品种中不存在，因此它现在可以用于提高一系列品种对条锈病的抗性。
Stripe rust is a devastating fungal disease that afflicts wheat in many regions of the world. New races of Puccinia striiformis, the pathogen responsible for this disease, have overcome most of the known race-specific resistance genes. We report the map-based cloning of the gene Yr36 (WKS1), which confers resistance to a broad spectrum of stripe rust races at relatively high temperatures (25 degrees to 35 degrees C). This gene includes a kinase and a putative START lipid-binding domain. Five independent mutations and transgenic complementation confirmed that both domains are necessary to confer resistance. Yr36 is present in wild wheat but is absent in modern pasta and bread wheat varieties, and therefore it can now be used to improve resistance to stripe rust in a broad set of varieties.