Resistance to Bemisia tabaci in tomato wild relatives
Resistance to Bemisia tabaci in tomato wild relatives
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DOI：
10.1007/s10681-012-0704-2
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发表时间：
2012-09-01
期刊：
影响因子：
1.9
通讯作者：
Vosman, Ben
中科院分区：
文献类型：
--
作者：
Firdaus, Syarifin;van Heusden, Adriaan W.;Vosman, Ben
烟粉虱是农业中最具威胁性的害虫之一，特别是在露地栽培的茄科作物如番茄和辣椒中。农药的使用往往无效，而且对人类和环境有害。利用存在于番茄野生亲缘植物中的植物天然防御，可能会提供一个解决方案。为了评估抗性参数，并确定具有高水平的抗性的植物材料，我们使用三种方法筛选了一些番茄野生近缘种的加入;在印度尼西亚的一个屏蔽室中进行的自由选择测试，在温室中用夹子笼进行的无选择测试和在荷兰的气候室中进行的叶盘测试。抗生素导致低成株存活率是番茄抗性的主要组成部分。然而，其他阻力分量也可以起作用。在一些加入有一个变化，随着时间的推移，抗性水平。在不同的测试中使用的几个电阻参数有很好的相关性。最好的抗性来源是加入的茄galapagense，这在过去没有被确定为具有抗性。这是特别令人感兴趣的，因为该物种与栽培番茄密切相关，这可能有助于抗性组分的渗入。粉虱的非偏好性和抗性与IV型毛状体的存在相关。其他机制可能涉及，因为一些加入没有IV型毛状体若虫密度低。叶盘试验是夹式笼粉虱抗性筛选的良好体外替代方法，如该试验和夹式笼试验所得结果之间的高度相关性所示。这使育种者能够更有效地进行测试。
Bemisia tabaci is one of the most threatening pests in agriculture, particularly in Solanaceous crops such as tomato and pepper that are cultivated in the open field. Pesticide application is often not effective and hazardous to humans and environment. The exploitation of plant natural defenses that are present in wild relatives of tomato, may offer a solution. To evaluate resistance parameters and to identify plant material with high levels of resistance, we screened a number of accessions of tomato wild relatives using three methods; a free-choice test in a screenhouse in Indonesia, a no-choice test with clip-on cages in a greenhouse and a leaf disc test in a climate-room in the Netherlands. Antibiosis resulting in low adult survival was the major component for resistance in tomato. However, other resistance component(s) may play a role as well. In some accessions there was a change in the resistance level over time. Several resistance parameters used in the different tests were well correlated. The best resistance source was an accession of Solanum galapagense, which had not been identified as being resistant in the past. This is of particular interest as this species is closely related to the cultivated tomato, which may facilitate introgression of the resistance component(s). Whitefly non-preference and resistance were associated with the presence of type IV trichomes. Other mechanisms might be involved since some accessions without type IV trichomes showed low nymphal density. The leaf disc test is a good in vitro alternative for the clip-on cage whitefly resistance screening, as shown by the high correlation between the results obtained with this test and the clip-on cage test. This offers breeders the possibility to carry out tests more efficiently.