LESION FORMATION AND LESION REMINERALIZATION IN ENAMEL UNDER CONSTANT COMPOSITION CONDITIONS - A NEW TECHNIQUE WITH APPLICATIONS
LESION FORMATION AND LESION REMINERALIZATION IN ENAMEL UNDER CONSTANT COMPOSITION CONDITIONS - A NEW TECHNIQUE WITH APPLICATIONS
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DOI：
10.1159/000260887
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发表时间：
1985-01-01
期刊：
影响因子：
4.2
通讯作者：
ARENDS, J
中科院分区：
文献类型：
--
作者：
BUSKES, JAKM;CHRISTOFFERSEN, J;ARENDS, J
本文介绍了一种新的在恒定成分条件下进行离体牙釉质脱矿的装置.去矿化或去矿化溶液以受控的速率流过试样。在人工损伤形成过程中（使用含有甲基羟基二膦酸盐（MHDP）的醋酸盐缓冲液），溶液的组成是恒定的。钙和磷酸盐的变化小于3%，pH值的变化小于0.03 pH单位。对于釉质，这些变化在釉质样本之间的生物学变化内。实验上发现，流过釉质表面的液体不能从表面上除去可测量的材料（腐蚀），实验误差为0.1 μ m。这是通过在脱矿过程中的显微硬度压痕来测量的。在2-50 μ M的范围内研究了各种MHDP浓度对脱矿作用的影响。即使是低浓度的MHDP证明是有效的显微硬度增加和损伤深度减少在脱矿。在脱矿实验中，通过显微放射照相术、硬度测量以及光学显微镜显示，所述方法快速产生可再现的损伤。在脱矿釉质的矿化实验中，病变的有效矿化发生在360小时内，使显微硬度增加三倍，病变深度减少两倍。
A new apparatus for the de- amd remineralization of enamel in vitro under constant composition conditions is described. A de- or remineralizing solution flows over the specimens at a controlled rate. During artificial lesion formation (using an acetate buffer containing methylhydroxydiphosphonate, MHDP), the composition of the solution is constant. The Ca and phosphate variation is less than 3%, pH variation is less than 0.03 pH unit. For enamel these variations are within the biological variations between enamel specimens. Experimentally it was found that the liquid streaming over the enamel surface does not measurably remove material from the surface (erosion) with the experimental error of 0.1 .mu.m. This was measured by following microhardness indentations during dimineralization. The effect of various MHDP concentrations on demineralization was investigated in the range of 2-50 .mu.M. Even low concentrations of MHDP prove to be effective in the microhardness increase and lesion depth reduction during demineralization. In the demineralization experiment it is shown by means of microradiography, hardness measurements as well as light microscopy that the method described quickly produces reproducible lesions. In a remineralization experiment of demineralized enamel effective remineralization of the lesions occurs in 360 h, giving a threefold increase in microhardness and a twofold decrease in lesion depth.