Resveratrol Oligomers Isolated from Carex Species Inhibit Growth of Human Colon Tumorigenic Cells Mediated by Cell Cycle Arrest
Resveratrol Oligomers Isolated from Carex Species Inhibit Growth of Human Colon Tumorigenic Cells Mediated by Cell Cycle Arrest
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DOI：
10.1021/jf201561e
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发表时间：
2011-08-24
影响因子：
6.1
通讯作者：
Henry, Geneive E.
中科院分区：
文献类型：
--
作者：
Gonzalez-Sarrias, Antonio;Gromek, Samantha;Henry, Geneive E.
研究表明，Cares属的成员产生生物活性的芪类化合物，包括白藜芦醇低聚物。这是非常感兴趣的营养品行业，因为白藜芦醇，葡萄和红葡萄酒的成分，已经吸引了巨大的研究关注，由于其潜在的人类健康的好处。在本研究中，评价了五种白藜芦醇低聚物（分离自苔草（Carex folliculata）和苔草（Carex gynandra））与白藜芦醇一起沿着对人结肠癌（HCT-116、HT-29、Caco-2）和正常人结肠（CCD-18 Co）细胞的抗增殖作用。白藜芦醇低聚物包括一个二聚体、两个三聚体和两个四聚体：分别为pallidol（1）、α-viniferin（2）和trans-miyabenol C（3）以及kobphenols A（4）和B（5）。虽然没有细胞毒性，但白藜芦醇寡聚物（1-5）以及白藜芦醇抑制人结肠癌细胞的生长。在六种芪类化合物中，α-葡萄苷（2）对结肠癌细胞最有效，IC 50值为6-32 μ M（与正常结肠细胞相比>2倍）。此外，α-葡萄苷（在20 μ M）不诱导细胞凋亡，但逮捕细胞周期（在S期）的结肠癌，但不是正常的结肠细胞。这项研究增加了越来越多的知识支持白藜芦醇及其低聚物的抗癌作用。此外，应研究Cares物种的营养潜力，因为它们产生生物活性的芪类化合物，如α-葡萄苷。
Research has shown that members of the Cares genus produce biologically active stilbenoids including resveratrol oligomers. This is of great interest to the nutraceutical industry given that resveratrol, a constituent of grape and red wine, has attracted immense research attention due to its potential human health benefits. In the current study, five resveratrol oligomers (isolated from Carex folliculata and Carex gynandra), along with resveratrol, were evaluated for antiproliferative effects against human colon cancer (HCT-116, HT-29, Caco-2) and normal human colon (CCD-18Co) cells. The resveratrol oligomers included one dimer, two trimers, and two tetramers: pallidol (1); alpha-viniferin (2) and trans-miyabenol C (3); and kobophenols A (4) and B (5), respectively. Although not cytotoxic, the resveratrol oligomers (1-5), as well as resveratrol, inhibited growth of the human colon cancer cells. Among the six stilbenoids, alpha-viniferin (2) was most active against the colon cancer cells with IC50 values of 6-32 mu M (>2-fold compared to normal colon cells). Moreover, alpha-viniferin (at 20 mu M) did not induce apoptosis but arrested cell cycle (in the S-phase) for the colon cancer but not the normal colon cells. This study adds to the growing body of knowledge supporting the anticancer effects of resveratrol and its oligomers. Furthermore, Cares species should be investigated for their nutraceutical potential given that they produce biologically active stilbenoids such as alpha-viniferin.