Growth hormone pretranslationally regulates the sexually dimorphic expression of the prolactin receptor gene in rat liver.
Growth hormone pretranslationally regulates the sexually dimorphic expression of the prolactin receptor gene in rat liver.
复制标题
生长激素翻译前调节大鼠肝脏中催乳素受体基因的性二态性表达。
作者：
J. Robertson;L. Haldosen;T. Wood;M. Steed;J. Gustafsson
大鼠肝脏 PRL 受体 (PRL-R) 基因的雌性特异性表达通过对垂体切除的大鼠进行 Northern 分析来研究，这些大鼠经过两种替代的人类 GH 治疗，模拟 1) 连续的雌性特异性或 2) 不连续的雄性特异性血清 GH 模式。前一种（女性特异性）模式被证明会导致男性和女性中 PRL-R mRNA 的急剧增加，而后一种（男性特异性）模式未能引起这种反应。连续施用牛 GH 后，在垂体切除的雌性动物中显示出类似的诱导效应，并且发现 PRL-R mRNA 水平增加了约 60 倍。牛 GH 的这种效应与人类同工型不同，缺乏催乳特性，因此表明了导致这种诱导反应的信号的体源起源。这些观察结果与先前获得的其他 GH 调节非受体基因的数据相结合，被解释为支持 GH 血清模式是性别特异性基因表达翻译前调节的更一般机制中的早期信号的提议。与 GH 相比，配体绵羊 PRL 仅引起 PRL-R mRNA 的轻微升高，而绵羊 PRL 与牛 GH 的共同施用未能提高单独使用牛 GH 时发现的 mRNA 水平。在连续施用 GH 后，除了主要的 2.2 千碱基形式之外，在肝脏中还检测到了先前未报告的约 3.0、3.8 和 5 千碱基的 PRL-R mRNA。
The female-specific expression of the rat liver PRL receptor (PRL-R) gene was investigated by Northern analysis of hypophysectomized rats after two alternative human GH treatments that were to mimic either 1) the continuous female-specific or 2) the discontinuous male-specific serum GH patterns. The former (female-specific) pattern was shown to result in a dramatic increase in PRL-R mRNA in both males and females, while the latter (male-specific) pattern failed to evoke this response. A similar inductive effect in hypophysectomized females was shown after continuous administration of bovine GH and was found to constitute an approximately 60-fold increase in PRL-R mRNA levels. This effect by bovine GH, which, unlike the human isoform, is devoid of lactogenic properties, thus indicates the somatogenic origin of the signal resulting in this inductive response. These observations in conjunction with previous data obtained for other GH-regulated nonreceptor genes are interpreted to support the proposal of GH serum patterns being an early signal in a more general mechanism for pretranslational regulation of sex-specific gene expression. In contrast to GH, only a slight elevation of PRL-R mRNA was evoked by the ligand ovine PRL, while coadministration of ovine PRL with bovine GH failed to enhance the mRNA level found with bovine GH alone. The detection of previously unreported PRL-R mRNAs in liver of approximately 3.0, 3.8, and 5 kilobases in addition to the major 2.2-kilobase form was also evident after continuous GH administration.
生长激素与大鼠脂肪细胞表面受体的共价结合。
影响因子：
4.8
作者：
Gorin,E;Goodman,HM
通讯作者：
Goodman,HM
小鼠肝膜中生长激素受体异质群体的鉴定和表征。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Smith,WC;Talamantes,F
通讯作者：
Talamantes,F
大鼠肝细胞生长激素受体的结构和蛋白水解。
影响因子：
2.9
作者：
Yamada,K;Lipson,KE;Donner,DB
通讯作者：
Donner,DB