SPECIFIC ABSORPTION OF LYMPHOCYTOTOXIC ALLOANTIBODIES BY THE LIVER IN INBRED RATS
SPECIFIC ABSORPTION OF LYMPHOCYTOTOXIC ALLOANTIBODIES BY THE LIVER IN INBRED RATS
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DOI：
10.1097/00007890-199008000-00027
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发表时间：
1990-08-01
期刊：
影响因子：
6.2
通讯作者：
BISMUTH, H
中科院分区：
文献类型：
--
作者：
GUGENHEIM, J;AMOROSA, L;BISMUTH, H
已经表明，肝移植物对淋巴细胞毒性抗体的敏感性低于其他器官移植物。在致敏近交系大鼠受体的实验研究中，我们使用体外肝脏血液灌流来研究肝脏和淋巴细胞毒性抗体之间的相互作用。供体特异性肝脏血液灌流可延缓心脏移植超急性排斥反应，降低淋巴细胞毒性抗体水平。免疫荧光检查的血液灌流肝脏发现存款的枯否细胞和IgG的窦细胞上的C3。在对照组大鼠中，第三方肝脏，供体特异性脾或肾血液灌流，心脏移植物存活时间延长较少。抗体水平的下降不显著，C3和IgG的存款也不明显。类似地，先前通过硫酸葡聚糖阻断供体特异性血液灌流肝脏的枯否细胞抑制了肝脏血液灌流的效果。这些结果支持了这一假设，即肝脏对超急性排斥反应的抵抗可能是由于大量和无毒的淋巴细胞毒性抗体吸收到非实质肝细胞上。
It has been suggested that liver allografts are less sensitive to lymphocytotoxic antibodies than other organ allografts. In this experimental study in sensitized inbred rat recipients, we have used extracorporeal liver hemoperfusion to study interactions between the liver and lymphocytotoxic antibodies. Donor-specific liver hemoperfusion can delay hyperacute rejection of heart allografts and reduce the level of lymphocytotoxic antibodies. Immunofluorescence examination of the hemoperfused liver revealed deposits of C3 on Kupffer cells and of IgG on sinusoidal cells. In control rats in which a third-party liver, a donor-specific splenic or renal hemoperfusion was peformed, heart allograft survival was less prolonged. The decrease in antibody levels was not significant and the deposit of C3 and IgG was much less evident. Similarly, previous blockade of the Kupffer cells of the donor-specific hemoperfused liver by dextran sulfate suppressed the effect of liver hemoperfusion. These results support the hypothesis that resistance of the liver to hyperacute rejection might be due to a massive and nontoxic absorption of lymphocytotoxic antibodies onto nonparenchymal liver cells.