Antifungal activity of cerebrospinal fluid against Cryptococcus neoformans and Candida species
Antifungal activity of cerebrospinal fluid against Cryptococcus neoformans and Candida species
复制标题
DOI：
10.1046/j.1365-280x.1999.00240.x
复制
发表时间：
1999-10-01
期刊：
影响因子：
2.9
通讯作者：
Stevens, DA
中科院分区：
文献类型：
--
作者：
Brummer, E;Drasin, T;Stevens, DA
在RPMI-1640中尝试了人脑脊液（CSF）对新生隐球菌和念珠菌生长的影响。单独CSF对两种酵母菌都具有高度抑真菌作用，并以浓度依赖性方式抑制生长。与人血清不同，CSF与氟康唑对C.新人类在G-200 Sephadex柱上对CSF进行分子筛分级分离，得到分子量约为66 kDa的高度抗真菌级分。在SDS-PAGE上，这部分迁移作为一个主要的和一个次要的带对应的牛血清白蛋白的流动性。这些新的发现表明CSF中含有一种因子，可以抵抗C.新生菌或念珠菌属。
The effect of human cerebrospinal fluid (CSF) on the growth of Cryptococcus neoformans and Candida species was tasted in RPMI-1640. CSF alone was highly fungistatic for both yeasts and inhibited growth in a concentration-dependent manner. Unlike human serum, CSF did not collaborate with fluconazole for killing C. neoformans. Molecular sieve fractionation of CSF on a G-200 Sephadex column yielded a highly antifungal fraction with a molecular weight around 66 kDa. On SDS-PAGE this fraction migrated as a major and a minor band corresponding to the mobility of bovine serum albumin. These novel findings suggest that CSF contains a factor(s) that provides resistance to the growth of C. neoformans or Candida species.