Complete genome sequence of a novel lytic bacteriophage isolated from Ralstonia solanacearum
Complete genome sequence of a novel lytic bacteriophage isolated from Ralstonia solanacearum
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DOI：
10.1007/s00705-017-3555-2
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发表时间：
2017-09
影响因子：
2.7
通讯作者：
J. Su;Hongwei Sun;Junjie Liu;Zhaokui Guo;G. Fan;Gang Gu;Guanghua Wang
中科院分区：
文献类型：
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作者：
J. Su;Hongwei Sun;Junjie Liu;Zhaokui Guo;G. Fan;Gang Gu;Guanghua Wang
用青枯拉尔氏菌Tb15-14从福建省中国南部烟田土壤中分离到一株裂解噬菌体RSPI1。使用高通量的Ion Torrent PGM测序仪对该噬菌体进行全基因组测序。RSPI1全基因组全长43,211个核苷酸，DNA G+C含量平均为61.5%。共鉴定出48个开放阅读框，长度在132~5,061 bp之间，其中11个为功能基因，12个为结构基因，25个为未知基因。BLAST分析表明，该噬菌体基因组的查询覆盖率为78-79%，与4个感染假鼻疽伯克霍尔德氏菌的足噬菌体的最高同源性为84%。利用噬菌体DNA聚合酶I和尾部纤维蛋白序列构建了两个相邻的系统发育树，表明该噬菌体与伯克霍尔噬菌体BP-AMP1密切相关，也与感染青枯雷尔氏菌的几个噬菌体有亲缘关系。这些发现表明，RSPI1是一种新的噬菌体，可以感染臭名昭著的植物病原菌青枯病。
A lytic podophage RSPI1 was isolated from tobacco field soil collected in Fujian Province, South China using host bacteriumRalstonia solanacearumTb15-14. Whole genome sequencing of this phage was performed using the high-throughput Ion Torrent PGM Sequencer. The complete genome of RSPI1 was 43,211 bp in length with a mean DNA G + C content of 61.5%. A total of 48 open reading frames were identified with lengths ranging from 132 bp to 5,061 bp, of which, 11, 12 and 25 were identified as functional, structural and unknown genes, respectively. A BLAST analysis revealed that this phage genome had a query cover of 78–79% and a highest identity of 84% with four podophages that infectBurkholderia pseudomallei. Two neighbor-joining phylogenetic trees were constructed using phage DNA polymerase I and tail fiber protein sequences and showed that this phage is closely related toBurkholderiaphage Bp-AMP1, and also related to several phages that infectRalstonia solanacearum. These findings indicate that RSPI1 is a novel phage that infects the notorious plant pathogenRalstonia solanacearum.